The drug hypoxen: A new inhibitor of mitochondrial respiration and dehydrogenases

Abstract

The effect of hypoxen on the oxygen consumption and activity of dehydrogenases in rat liver mitochondria has been studied. The addition of hypoxen to mitochondria caused a reduction of the rate of phosphorylating and uncoupling respiration. The minimal effective concentration of hypoxen was 15 μg/ml with succinate, 60 μg/ml with pyruvate or palmitoylcarnitine, and 120 μg/ml with glutamate as the substrates. The activities of malate, glutamate, and succinate dehydrogenases in mitochondria were significantly decreased by the effect of hypoxen.

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References

  1. Eropkin, M.Yu., Gudkova, T.M., Konovalova, N.I., et al., Antiviral Effect of Some Antioxidants/Antihypoxants and Their Combination with Remantadin against Human Influenza Virus A(H3N2) Studied in Models in vitro, Eksperim. Klin. Farmakol., 2007, vol. 70, no. 5, pp. 33–37.

    CAS  Google Scholar 

  2. Kochetygov, N.I. and Makeev, A.B., Infusion Therapy of Experimental Ozone Shock using Antihypoxant Olifen, Patol. Fiziol. Eksp. Ter., 1992, no. 5–6, pp. 32–34.

  3. Kosenko, E., Felipo, V., Montoliu, C., et al., Effects of Acute Hyperammonemia in vivo on Oxidative Metabolism in Nonsynaptic Rat Brain Mitochondria, Metab. Brain Dis., 1997, vol. 12, pp. 69–82.

    Article  CAS  PubMed  Google Scholar 

  4. Kosenko, E.A., Comparative Study of Energy Metabolism in the Liver and Skeletal Muscle of Rat and Rabbit: Effect of Starvation, Biokhimiya, 1981, vol. 46, pp. 1389–1395.

    CAS  Google Scholar 

  5. Lowry, O.H., Rosenbrough, N.J., Farr, A.L., and Randall, R.J., Protein Measurement with the Folin Phenol Reagent, J. Biol. Chem., 1951, vol. 193, pp. 265–275.

    CAS  PubMed  Google Scholar 

  6. Rasmussen, U.F. and Rasmussen, H.N., Human Quadriceps Muscle Mitochondria: a Functional Characterization, Mol. Cell Biochem., 2000, vol. 208, pp. 37–44.

    Article  CAS  PubMed  Google Scholar 

  7. Semigolovskii, N.Yu., Application of Antihypoxants in Acute Myocardial Infarction, Anesteziol. Reanimatol., 1998, no. 2, pp. 56–59.

  8. Smirnov, A.V. and Krivoruchko, B.I., Antihypoxants in Emergency Medicine, Anesteziol. Reanimatol., 1998, no. 2, pp. 50–55.

  9. Smirnov, V.S. and Kuz’mich, M.K., Gipoksen (Hypoxen), St. Petersburg: FARMindeks, 2001.

    Google Scholar 

  10. Tolstoi, A.D., Dzhurko, B.I., Vashetko, R.V., et al., Histoprotective Effect of Antihypoxant Olifen in Experimental Acute Pancreatitis, Byull. Eksp. Biol. Med., 2001, vol. 131, pp. 312–314.

    Article  CAS  Google Scholar 

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Correspondence to Yu. G. Kaminskii.

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Original Russian Text © E.A. Kosenko, M.B. Abramova, N.I. Venediktova, I.I. Popova, Yu.G. Kaminskii, 2010, published in Izvestiya Akademii Nauk, Seriya Biologicheskaya, 2010, No. 4, pp. 411–416.

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Kosenko, E.A., Abramova, M.B., Venediktova, N.I. et al. The drug hypoxen: A new inhibitor of mitochondrial respiration and dehydrogenases. Biol Bull Russ Acad Sci 37, 346–350 (2010). https://doi.org/10.1134/S1062359010040035

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Keywords

  • Biology Bulletin
  • Mitochondrial Respiration
  • Minimal Effective Concentration
  • Palmitoylcarnitine
  • Succinate Oxidation