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Molecular structure of allelic variants of microsatellite loci Du281 and Du47 in unisexual and bisexual lizard species of the genus Darevskia

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Abstract

Experimental data on the molecular structure and variability of microsatellite loci in unisexual and bisexual lizard species of the genus Darevskia were analyzed. The allelic variants of Du281 and Du47 were found to differ in the number of monomers, the structure of microsatellite clusters, and point mutations in these clusters and flanking DNA. Interspecific comparison of alleles of these loci revealed both variable regions in the microsatellite clusters and allele-specific evolutionarily conserved nucleotide groups. In general, the results of comparative structural analysis of allelic variants testify to a high genetic similarity of the unisexual and bisexual lizard species studied and reveals the characteristic features of their interspecies variability.

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Correspondence to V. I. Korchagin.

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Original Russian Text © A.P. Ryskov, I.A. Martirosyan, A. A. Vergun, D.N. Malysheva, T.N. Badaeva, O.N. Tokarskaya, N.S. Vassetzky, V.I. Korchagin, 2009, published in Izvestiya Akademii Nauk, Seriya Biologicheskaya, 2009, No. 2, pp. 201–208.

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Ryskov, A.P., Martirosyan, I.A., Vergun, A.A. et al. Molecular structure of allelic variants of microsatellite loci Du281 and Du47 in unisexual and bisexual lizard species of the genus Darevskia . Biol Bull Russ Acad Sci 36, 159–166 (2009). https://doi.org/10.1134/S1062359009020083

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