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Isolation and purification of malate dehydrogenase isoforms from phototrophic purple bacteria Rhodobacter sphaeroides and Rhodopseudomonas palustris

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Abstract

A five-step procedure was used to obtain electrophoretically pure preparations of malate dehydrogenase (EC 1.1.1.37) from Rhodobacter sphaeroides and Rhodopseudomonas palustris. The procedure included extraction, ammonium sulfate fractionation, gel filtration, and ion exchange and gel permeation chromatography. The enzyme was found to exist in two isoforms, dimeric and tetrameric, formed by the oligomerization of identical subunits. The isoforms are assumed to be involved in different metabolic processes.

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Authors and Affiliations

  1. Voronezh State University, Universitetskaya pl. 1, Voronezh, 394006, Russia

    A. T. Eprintsev, M. A. Klimova & K. D. Shikhalieva

  2. Vinogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, Moscow, 117811, Russia

    E. I. Kompantseva

Authors
  1. A. T. Eprintsev
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  2. M. A. Klimova
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  3. K. D. Shikhalieva
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  4. E. I. Kompantseva
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Correspondence to A. T. Eprintsev.

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Original Russian Text © A.T. Eprintsev, M.A. Klimova, K.D. Shikhalieva, E.I. Kompantseva, 2008, published in Izvestiya Akademii Nauk, Seriya Biologicheskaya, 2008, No. 6, pp. 680–687.

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Eprintsev, A.T., Klimova, M.A., Shikhalieva, K.D. et al. Isolation and purification of malate dehydrogenase isoforms from phototrophic purple bacteria Rhodobacter sphaeroides and Rhodopseudomonas palustris . Biol Bull Russ Acad Sci 35, 585–591 (2008). https://doi.org/10.1134/S1062359008060046

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