Abstract
Accumulation of considerable amounts of viral particles has been demonstrated in parenchymal cells of young leaves in tobacco cultivar Samsun systemically infected with any of studied tobacco mosaic virus (TMV) strains isolated from pepper (TMV-p), tomato (TMV-t), and eggplant (TMV-e). Abnormal (swollen and thin) virions were found, which points to their destruction. Cell infection with all studied strains was accompanied by the activation of the lysosomal compartment manifested as formation of nascent dictyosomes, elements of smooth endoplasmic reticulum, cytoplasmic vacuoles, various vesicles, invaginated mitochondria, and multivesicular bodies. The studied viral strains could be arranged in the following sequence according to the degree of lysosomal compartment stimulation and induction of intracellular lytic processes mediating the destruction of viral particles and cell structures: TMV-p > TMV-e > TMV-t.
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References
Belitser, N.V., Lysosomal System and Microbodies in Plant and Animal Cells, Doctoral (Biol.) Dissertation, Leningrad, 1978.
Buzhoryanu, V.V., Ul’trastruktura rastitel’noi kletki pri virusnoi infektsii (Plant Cell Ultrastructure during Viral Infection), Chisinau: Shtiintsa, 1986.
Esau, K., Viruses in Plant Hosts. Form, Distribution and Pathologic Effect. Madisom, Milwaukee, L.: Univ. Wisconsin Press, 1968.
Everitt, E., Persson, M.J., and Wohlfart, C., pH-Dependent Exposure of Endoproteolytic Cleavage Sites of the Adenovirus 2 Hexon Protein, FEMS Microbiol. Lett., 1988, vol. 49, no. 2, pp. 229–233.
Gnutova, R.V., Tolkach, V.F., and Gnutova, I.V., Tobamoviruses: New TMV Strains, in Akual’nye problemy fitovirusologii i zashchity rastenii (Current Problems of Plant Virology and Plant Protection), Minsk: BelNII zashchity rastenii, 1997, pp. 40–41.
Kim, K.S. and Fulton, J.P., Fine Structure of Plant Cells Infected with Bean Pod Mottle Virus, Virology, 1972, vol. 49, no. 1, pp. 112–121.
Kursanov, A.L., Transport assimilyatov v rastenii (Assimilate Transport in Plants), Moscow: Nauka, 1976.
Mashanskii, V.F., Vinnichenko, L.N., and Mosevich, T.N., et al., Two Different Types of Ultrastructural Changes of Mitochondria in Animal and Plant Cells, in Ul’trastruktura rastitel’nykh kletok (Plant Cell Ultrastructure), Leningrad: Nauka, 1972, pp. 98–102.
Matthews, R.E.F., Plant Virology, London: Academic, 1970. Translated under the title Virusy rastenii, Moscow: Mir, 1973.
Matthews, R.E.F., Plant Virology. Second Edition, New York: Academic, 1981.
Mayee, C.D. and Sarkar, D.S., The Ultrastructure of Nicotiana tabacum Cells Infected with Potato Virus X and Potato Virus Y, J. Ultrastruct. Res., 1982, vol. 81, no. 1, pp. 124–131.
Nakagaki, Y. and Hirai, T., Effect of Detached Leaf Treatment on Tobacco Mosaic Virus Multiplication in Tobacco and Bean Leaves, Phytopathology, 1971, vol. 61, no. 1, pp. 22–27.
Oliveira, L., Self-Degeneration of Mitochondria in the Root Cap Cells of Triticale. Its Contribution to the Development of the Vacuolar Apparatus and Significance for Senescence, Caryologia, 1975, vol. 28, no. 4, pp. 511–523.
Reunov, A.V., Cytopathology of Plant Cell Infected with Viruses (TMV, PXV) and the Problem of Plant Resistance, Doctoral (Biol.) Dissertation, Kiev, 1989.
Reunov, A.V., Lapshina, L.A., and Reifman, V.G. Abnormal Mitochondria and Cells in the Basal Zone of Apical Tobacco Leaves Infected with TMV and Intact, Tsitologiya i genetika, 1986, vol. 20, no. 3, pp. 209–211.
Rogovin, V.V. and Fomina, V.A., Novel Autophagic Function of Mitochondria, Izv. Akad. Nauk SSSR. Ser. Biol., 1983, no. 6, pp. 923–924.
Russo, M. and Martelli, G.P., Ultrastructure of Turnip Crinkle-and Saguaro Cactus Virus-Infected Tissues, Virology, 1982, vol. 118, no. 1, pp. 109–116.
Saito, T., Hosokawa, D., Meshi, T., and Okada, Y., Immunocytochemical Localization of the 130 K and 180 K Proteins (Putative Replicase Components) of Tobacco Mosaic Virus, Virology, 1987, vol. 160, no. 2, pp. 477–481.
Vasil’ev, A.E., Funktsional’naya morfologiya sekretornykh kletok rastenii (Functional Morphology of Plant Secretory Cells), Leningrad: Nauka, 1977.
Vasil’ev, A.E., Problems of Endocytosis and Autophagy in Plant Cell, Ul’trastruktura rastitel’nykh kletok (Ultrastructure of Plant Cell), Leningrad: Nauka, 1972, pp. 3–60.
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Original Russian Text © A.V. Reunov, I.V. Gnutova, L.A. Lapshina, V.P. Nagorskaya, V.F. Tolkach, 2006, published in Izvestiya Akademii Nauk, Seriya Biologicheskaya, 2006, No. 4, pp. 502–509.
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Reunov, A.V., Gnutova, I.V., Lapshina, L.A. et al. Effect of tobacco mosaic virus strains on the ultrastructure of tobacco leaf parenchymal cells. Biol Bull Russ Acad Sci 33, 409–415 (2006). https://doi.org/10.1134/S1062359006040145
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DOI: https://doi.org/10.1134/S1062359006040145