Abstract
Chemical signal transduction from the cell surface to organelles was studied in unicellular vegetative (Equisetum arvense) and generative (Hippeastrum hybridum pollen) microspores of plants. Neurotransmitters acetylcholine, dopamine, and serotonin, their agonists and antagonists, Na+, K+, and Ca2+ channel blockers, as well as forskolin and theophylline (agents increasing the intracellular level of cyclic adenosine monophosphate) were used as chemical signals. Both types of microspores exposed to neurotransmitters, their agonists, forskolin, and theophylline demonstrated growth activation, while neurotransmitter antagonists and ion channel blockers inhibited this process. No stimulating effects of neurotransmitters were observed for cells pretreated with the antagonists and ion channel blockers. Pretreatment with ion channel blockers and then by anticontractile agents (cytochalasin B or colchicine) either had no effect or increased the inhibition of microspore growth. Pathways of chemical signal transduction from the cell surface to organelles are discussed.
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Andreev, I.M., Timofeev, G.V., and Kovaleva, L.V., Generation of Calcium Signal in Pollen Grains Triggered by Depolarization of the Plasma Membrane, Dokl. Akad. Nauk, 2005, vol. 400, no. 5, pp. 693–696.
Bednarska, E., The Effect of Exogenous Ca2+ Ions on Pollen Grain Germination and Pollen Tube Growth, Sex. Plant Reprod., 1989, vol. 2, no. 1, pp. 53–58.
Bezuglov, V.V., Gretskaya, N.M., Esipov, S.E., Polyakov, N.B., Nikitina, L.A., Buznikov, G.A., and Lauder, Dzh., Fluorescent Labeled Lipophilic Analogs of Serotonin, Dopamine, and Acetylcholine: Synthesis, mass-spectrometry, and Biological Activity, Bioorg. Khim., 2004, vol. 30, no. 5, pp. 512–519.
Buznikov, G.A., Neirotransmittery v embriogeneze (Neurotransmitters in Embryogenesis), Moscow: Nauka, 1987.
Cantiello, H.F., Role of Actin Filaments Organization in Cell Volume and Ion Channel Regulation, J. Exp. Zool., 1997, vol. 279, no. 2, pp. 425–435.
Franklin-Tong, V.E., Signaling and the Modulation of Pollen Tube Growth, Plant Cell, 1999, vol. 11, no. 3, pp. 727–738.
Franklin-Tong, V.E., Drobak, B.K., Allan, A.C., Watkins, P.A., and Trewavas, A.J., Growth of Pollen Tubes of Papaver rhoeas Is Regulated by a Slow-Moving Calcium Wave Propagated by Inositol 1,4,5-Triphosphate, Plant Cell, 1996, vol. 8, no. 8, pp. 1305–1321.
Groenewald, E.G. and van der Westhuizen, A.J., Prostaglandins and Related Substances in Plants, Bot. Rev., 1997, vol. 63, no. 3, pp. 199–220.
Hwang, J.-U., Suh, S., Yi, H., Kirn, J., and Lee, Y., Actin Filaments Modulate Both Stomatal Opening and Inward K +-Channel Activities in Guard Cells of Vicia faba L., Plant Physiol., 1997, vol. 115, no. 2, pp. 335–342.
Moutinho, A., Hussey, P.J., Trewavas, A.J., and Malho, R., cAMP Acts as a Second Messenger in Pollen Tube Growth and Reorientation, Proc. Natl. Acad. Sci. USA, 2001, vol. 98, no. 21, pp. 10481–10486.
Roshchina, V.V., Biomediatory v rasteniyakh. Atsetilkholin i biogennye aminy (Biomediators in Plants. Acetylcholine and Biogenic Amines), Pushchino: Biologicheskii Tsentr Akad. Nauk SSSR, 1991.
Roshchina, V.V., Chemical Signaling in Pollen, Usp. Sovrem. Biol., 1999, vol. 119, no. 6, pp. 557–566.
Roshchina, V.V., Neurotransmitters in Plant Life, Plymouth: Science, 2001.
Roshchina, V.V., Cellular Models to Study the Allelopathic Mechanisms, Allelopathy J., 2004a, vol 13, no. 1, pp. 3–16.
Roshchina, V.V., Plant Microspores as Unicellular Models for the Study of Relations between Contractile Components and Chemosignaling, Proc. Int. Symp. “Biological Motility”. Pushchino, 23 May–1 June 2004, Pushchino: ONTI, 2004b, pp. 194–196.
Roshchina, V.V., Contractile Proteins in Chemical Signal Transduction in Plant Microspores; Izv. Akad. Nauk. Ser. Biol., 2005a, no. 3, pp. 281–286.
Roshchina, V.V., Chemosignaling in Plant Microspores. Possible Role of Cytoskeleton, Proc. Int. Symp. “Reception and Intracellular Signaling”, Pushchino, 6–8 June 2005. Pushchino: ONTI, 2005b, pp. 399–402.
Roshchina, V.V., Popov, V.I., Novoselov, V.I., Mel’nikova, E.V., Gordon, R.Ya., Peshenko, I.V., and Fesenko, E.E., Chemical Signal Transduction in Pollen, Tsitologiya, 1998, vol. 40, no. 11, pp. 964–971.
Roshchina, V.V., Mel’nikova, E.V., Yashin, V.A., and Karnaukhov, V.N., Autofluorescence of Intact Equisetum arvense L. Spores during Their Development, Biofizika, 2002, vol. 47, no. 2, pp. 318–324.
Roshchina, V.V., Bezuglov, V.V., Markova, L.N., Sakharova, N.Yu., Buznikov, G.A., Karnaukhov, V.N., and Chailakhyan, L.M., Interaction of Living Cells with Fluorescent Derivatives of Biogenic Amines, Dokl. Ross. Akad. Nauk, 2003a, vol. 393, no. 6, pp. 832–835.
Roshchina, V.V., Miller, A.V., Safronova, V.G., and Karnaukhov, V.N., Reactive Oxygen Forms and Luminescence of Intact Microspore Cells, Biofizika, 2003b, vol. 48, no. 2, pp. 259–264.
Roshchina, V.V., Yashin, V.A., and Kononov, A.V., Autofluorescence of Plant Microspores Studied by Confocal Microscopy and Microspectrofluorimetry, J. Fluorescence, 2004, vol. 14, no. 6, pp. 745–750.
Staiger, C.J., Signaling to the Actin Cytoskeleton in Plant, Annu. Rev. Plant Physiol. Plant Mol. Biol., 2000, vol. 51, pp. 257–288.
Tret’yak, T.M. and Arkhipova, L.V., Intracellular Activity of Neuromediators, Usp. Sovrem. Biol., 1992, vol. 112, no. 2, pp. 265–272.
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Original Russian Text © V.V. Roshchina, 2006, published in Izvestiya Akademii Nauk, Seriya Biologicheskaya, 2006, No. 4, pp. 414–420.
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Roshchina, V.V. Chemical signaling in plant microspore cells. Biol Bull Russ Acad Sci 33, 332–338 (2006). https://doi.org/10.1134/S1062359006040030
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DOI: https://doi.org/10.1134/S1062359006040030