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Development of an UHPLC-UV Method for Quantification of Three Phenyl 4-(2-oxo-3-alkylimidazolidin-1-yl)benzenesulfonates in CD-1® IGS Female Mouse Plasma

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Abstract

Phenyl 4-(2-oxo-3-alkylimidazolidin-1-yl)benzenesulfonates (PAIB-SOs) are new antimitotic prodrugs bioactivated by the enzyme CYP1A1 into phenyl 4-(2-oxo-3-imidazolidin-1-yl)benzenesulfonates (PIB-SOs) that are potently (nM) and selectively inhibiting the growth of CYP1A1-expressing breast cancer cells. The screening program identified three PAIB-SOs designated as CEU-835, CEU-934, and CEU-938 for further pharmacokinetic studies. A novel method for their quantification in CD-1® IGS female mouse plasma using a Waters UHPLC ACQUITY Arc system was developed and validated. Their analytical measurements ranged from 7.3 to 1484 ng/mL. They exhibited linearity between 7.3 and 1484 ng/mL. They also showed good resolutions (2.21–4.99) and good theoretical plate numbers (14707–70580). Moreover, they were robust at wavelengths between 273 and 310 nm and sensitive from 2 to 62 AU/ng mL−1. This new method is indispensable to assess PAIB-SOs half-life in mouse plasma and for the quantification of PAIB-SOs in other biological matrices.

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ACKNOWLEDGEMENTS

The authors would like to thank Dr. Alexandre Patenaude for his assistance in the correction of the manuscript.

Funding

This research was supported by grants from Fonds de Recherche du Québec-Santé (FRQ-S), research infrastructure program of John R. Evans Leaders Fund from Canada Foundation for Innovation (CFI, Canada, grant No. 36231), Fonds d’enseignement et de recherche of the Faculté de pharmacie de l’Université Laval, Réseau québécois de recherche sur les médicaments (RQRM) and Canadian Institutes of Health Research (PJT-178096). A.C. Chavez Alvarez, C. Bouzriba, V. Ouellette and M. Gagné-Boulet are recipients of studentships from the Fonds d’enseignement et de recherche of the Faculté de pharmacie de l’Université Laval and FRQ-S. S. Fortin is the recipient of a Junior 2 research scholar award from FRQ-S.

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Authors and Affiliations

  1. Faculté de pharmacie, Université Laval, G1V 0A6, Québec, QC, Canada

    Atziri Corin Chavez Alvarez, Chahrazed Bouzriba, Laureline Bernier, Vincent Ouellette, Mathieu Gagné-Boulet, Chantale Simard & Sébastien Fortin

  2. Centre de recherche du CHU de Québec-Université Laval, G1L 3L5, Québec, QC, Canada

    Atziri Corin Chavez Alvarez, Chahrazed Bouzriba, Laureline Bernier, Vincent Ouellette, Mathieu Gagné-Boulet, René C.- Gaudreault & Sébastien Fortin

  3. Centre de Recherche de l’Institut universitaire de cardiologie et pneumologie de Québec-Université Laval, G1V 4G5, Québec, QC, Canada

    Atziri Corin Chavez Alvarez, Sylvie Pilote & Chantale Simard

  4. Département de médecine moléculaire, Faculté de médecine, Université Laval, G1V 0A6, Québec, QC, Canada

    René C.- Gaudreault

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  1. Atziri Corin Chavez Alvarez
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  2. Chahrazed Bouzriba
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  3. Laureline Bernier
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  4. Vincent Ouellette
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  6. Sylvie Pilote
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  7. René C.- Gaudreault
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  8. Chantale Simard
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  9. Sébastien Fortin
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Corresponding authors

Correspondence to Atziri Corin Chavez Alvarez or Sébastien Fortin.

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ETHICS APPROVAL AND CONSENT TO PARTICIPATE

Animal-related experiments were conducted in accordance with the Conseil canadien de protection des animaux (CCPA) and the Comité de protection des animaux de l’Université Laval (CPAUL) and its guidelines (https://www.services-recherche.ulaval.ca/conduite-responsable-et-ethique/protection-des-animaux). Animal protocols were approved by the CPAUL, Université Laval, Québec, Canada (Approval IDs 2018-014-1 and 2018-014-2).

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The authors of this work declare that they have no conflicts of interest.

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Alvarez, A., Bouzriba, C., Bernier, L. et al. Development of an UHPLC-UV Method for Quantification of Three Phenyl 4-(2-oxo-3-alkylimidazolidin-1-yl)benzenesulfonates in CD-1® IGS Female Mouse Plasma. J Anal Chem 79, 456–463 (2024). https://doi.org/10.1134/S1061934824040026

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  • DOI: https://doi.org/10.1134/S1061934824040026

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