Abstract
The present work describes the development of molecular iodine mediated facile and sensitive method for the spectrophotometric estimation of tolfenamic acid in pharmaceutical and veterinary formulations. Here, in the presence of iodine, the secondary amine of tolfenamic acid coordinates with the lone pair of available electrons with iodine, which results in the formation of hyper iodine complex of tolfenamic acid. The newly formed colored ion complex showed the absorbance maximum at 359 nm. The method was validated as recommended in ICH guidelines. The method was linear over the concentration range of 0.01–0.07 µg/mL (r2 = 0.9998) with the limits of detection and quantitation of 0.002 and 0.006 µg/mL, respectively. Intra-day (mean recovery of 99.7%) and inter-day (mean recovery of 100.9%) method precision was determined. The mean recovery of the accuracy study is between 99.0–100.5%. The method was found to be robust and suitable for assay of tolfenamic acid in pharmaceutical and veterinary formulations.
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REFERENCES
Jaunetta, H. and Nasir, S., Cells, 2021, vol. 10, no. 3, p. 702.
PubChem Substance Database. CID 610479 (Tolfenamic acid), National Centre for Biotechnology Information, version 5.0. Accessed October 04, 2017.
Evanson, N., Enna, S., and Bylund, D., The Comprehensive Pharmacology Reference, NewYork: Elsevier, 2007, p. 1.
Sweetman, S., Martindale: The Complete Drug Reference, London: Pharm. Press, 2009, 36th ed., p. 96.
Feldman, D., Leahy, E., and Lee Seong-Ho., Curr. Med. Chem., 2018, vol. 25, no. 14, p. 1598.
Subaiea, G., Ahmed, A., Adwan, L. and Zawia, N., J. Alzheimers Dis., 2014, vol. 43, no. 2, p. 425.
Niopas, I. and Georgarakis, M., J. Liq. Chromatogr., 1995, vol. 18, no. 13, p. 2675.
Rozou, S., Michaleas, S., and Antoniadou-Vyza, E., J. Pharm. Pharmacol., 2010, vol. 5, no. 2, p. 79.
British Pharmacopoeia, Monograph on Tolfenamic Acid, London: Her Majesty’s Stationary Office, 2016.
De Melo da Silva, L., Cavalcante, R., Cunha, R., Dantas, R., Oliveira, S., and Junior, A., Sci. Total Environ., 2016, vol. 573, p. 518.
Ahmed, S., Mustaan, N., Sheraz, M., Syeda Ayesha Ahmed un Nabi, and Ahmad, I., J. Pharm., 2015, vol. 2015, p. 216249.
Ahmed, S., Mustaan, N., Sheraz, M., Nabi, S., and Ahmad, I., Cent. Eur. J. Chem., 2013, vol. 11, no. 9, p. 1533.
Papadoyannis, I., Georgarakis, M., Samanidou, V., and Zotou, A., J. Liq. Chromatogr. Relat. Technol., 1991, vol. 14, p. 2951.
Gallo, P., Fabbrocino, S., Dowling, G., Salini, M., Fiori, M. and Perretta, G., J. Chromatogr. A, 2010, vol. 1217, p. 2832.
Nielsen-Kudsk, F., Acta Pharmacol. Toxicol., 1980, vol. 47, p. 267.
González-Barreiro, C., Lores, M., Casais, M.C., and Cela, R., J. Chromatogr. A, 2003, vol. 993, p. 29.
Mikami, E., Goto, T., Ohno, T., Matsumoto, H., Inagaki, K., and Ishihara, H., J. Chromatogr. B: Biomed. Sci. Appl., 2000, vol. 744, p. 81.
Rozou, S. and Antoniadou-Vyza, E., J. Pharm. Biomed. Anal., 1998, vol. 18, p. 899.
Niopas, I. and Georgarakis, M., J. Liq. Chromatogr. Relat. Technol., 1995, vol. 18, p. 2675.
Papadoyannis, N., Zotou, A., and Samanidou, V., J. Liq. Chromatogr. Relat. Technol., 1992, vol. 15, p. 1923.
Fernandes, A. and Pai, S., Int. J. Appl. Pharm., 2019, vol. 11, no. 5, p. 264.
Maurer, H., Tauvel, F., and Kraemer, T., J. Anal. Toxicol., 2001, vol. 25, p. 237.
Muñoz, V., Talio, M.C., Gil, R., and Fernandez, L.P., Talanta, 2020, vol. 207, p. 120345.
Andleeb, S., Ahemad, S., Sheraz, M., Anwar, Z., and Ahmad, I., Luminescence, 2020, vol. 35, no. 7, p. 1017.
El-Kafrawy, D., Abo-Gharam, A., Abdel-Khalek, M., Mahrous, M., and Belal, T., Res. J. Pharm., Biol. Chem. Sci., 2018, vol. 9, no. 2, p. 669.
Swamy, N. and Basavaiah, K., Braz. J. Pharm. Sci., 2014, vol. 50, no. 4, p. 839.
Abdulrahman, S., Devi, O., Basavaiah, K., and Vinay, K., J. Taibah Univ. Sci., 2015, vol. 10, no. 2016, p. 80.
Vinod Kumar, T., Seethamma, M., and Venkateshwarlu, G., J. Appl. Chem., 2014, vol. 7, no. 5, p. 7.
ICH, (Q2R1), Harmonized Tripartite Guideline, Validation of Analytical Procedures: Text and Methodology, Geneva: IFPMA, 2005.
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We are heartly thankful to Department of Higher Education, Government of Gujarat for providing SHODH scholarship to Hardik L. Varu.
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Varu, H., Kapuriya, N.P., Bhalodia, J.J. et al. Molecular Iodine Mediated Spectrophotometric Determination of Tolfenamic Acid in Pharmaceutical and Veterinary Formulations. J Anal Chem 78, 687–693 (2023). https://doi.org/10.1134/S1061934823060114
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DOI: https://doi.org/10.1134/S1061934823060114