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Highly Sensitive Determination of Carbamazepine and Oxcarbazepine and Identification of Their Degradation Products in Material Evidences and Human Cadaveric Liver by Gas Chromatography–Mass Spectrometry

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Abstract

The work is devoted to the search for conditions for the sample preparation and determination of carbamazepine and oxcarbazepine and identification of the products of their metabolism and degradation in human liver (post mortem) and material evidences by gas chromatography–mass spectrometry. A QUECHERS approach was developed to the sample preparation of carbamazepine and oxcarbazepine. Amitriptyline was proposed as an internal standard. Degradation products of carbamazepine and oxcarbazepine were studied in model solutions upon alkaline and acid hydrolysis and oxidation; 14 metabolites and degradation products were identified. The main analytical characteristics of the developed procedure were determined. The limits of detection are 0.1 and 0.2 µg/g for carbamazepine and oxcarbazepine, respectively. The developed procedure complies with the Validation Guidelines of the Russian Center for Forensic Medical Examination.

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ACKNOWLEDGMENTS

The study was carried out using the equipment of the Central Collective Use Center of Moscow State University “Technologies for Obtaining New Nanostructured Materials and Their Comprehensive Study”, acquired by Moscow State University under the program for updating the instrumentation base within the framework of the national project “Science” and the Development Program of Moscow State University.

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Authors and Affiliations

  1. Department of Chemistry, Moscow State University, 119991, Moscow, Russia

    A. V. Pirogov, N. A. Gandlevskii & A. A. Vasil’eva

  2. Russian Center of Forensic Expertise, Ministry of Health of the Russian Federation, 125284, Moscow, Russia

    S. S. Barsegyan

  3. Sechenov First Moscow State Medical University, 119048, Moscow, Russia

    A. E. Nosyrev

Authors
  1. A. V. Pirogov
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  2. N. A. Gandlevskii
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  3. A. A. Vasil’eva
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  4. S. S. Barsegyan
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  5. A. E. Nosyrev
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Correspondence to A. V. Pirogov.

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Translated by O. Zhukova

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Pirogov, A.V., Gandlevskii, N.A., Vasil’eva, A.A. et al. Highly Sensitive Determination of Carbamazepine and Oxcarbazepine and Identification of Their Degradation Products in Material Evidences and Human Cadaveric Liver by Gas Chromatography–Mass Spectrometry. J Anal Chem 78, 783–793 (2023). https://doi.org/10.1134/S1061934823060096

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