Abstract
Common antiepileptic drugs have complex pharmacokinetic characteristics leading to fluctuation in their plasma levels at the same therapeutic doses. Therefore, antiepileptic drugs turn out to be among the most common medications for which therapeutic drug monitoring (TDM) is crucial. Indeed, TDM provides a realistic approach to adjust drug doses in epilepsy care based on plasma concentrations to optimize its clinical outcome. Perampanel (PER) is an antiepileptic drug used for the treatment of primary generalized tonic-clonic seizures in combination with other drugs, such as carbamazepine (CAR). Drug-drug pharmacokinetic interactions are very common in this combination, which makes TDM of PER essential. A selective, accurate, and precise bioanalytical method has been developed for the simultaneous determination of PER and CAR in human plasma for the purpose of TDM. Liquid-liquid extraction using ethyl acetate was applied for sample preparation, and diazepam (DZP) was the internal standard. The adopted method could successfully determine PER and CAR within their cmax levels as the linearity range was 0.2–10 µg/mL for PER and 5–100 µg/mL for CAR. The chromatographic separation was achieved on a C8 column using acetonitrile, aqueous 0.1% glacial acetic acid (75 : 25, v/v) as a mobile phase in isocratic elution at a flow rate of 0.8 mL/min and an UV detection at 225 nm. The adopted method was validated according to EMA guidelines, and the results were within the acceptance criteria.
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REFERENCES
Tremblay, G., Barghout, V., Patel, V., Tsong, W., and Wang, Z., Epilepsy Behav., 2017, vol. 68, p. 196.
Epilepsies: Diagnosis and Management, London: Natl. Inst. Health Care Excellence, 2012.
Jacob, S. and Nair, A.B., Drugs R&D, 2016, vol. 16, no. 4, p. 303.
Brayfield, A., Matindale The Complete Drug Reference, London: Pharm. Press, 2014, 38th rd.
Brunton, L.L., Hilal-Dandan, R., and Knollmann, B.C., Goodman & Gilman’s: The Pharmacological Basis of Therapeutics, New York: McGraw-Hill, 2017, 13th ed.
US FDA, Fycompa® (Perampanel): Highlights of prescribing information, 2012. https://www.fycompa.com/-/media/Files/Fycompa/Fycompa_Prescribing_Information.pdf. Cited August 16, 2021.
St Louis, E.K., Rosenfeld, W.E., and Bramley, T., Curr. Neuropharmacol., 2009, vol. 7, no. 2, p. 77.
Kwan, P., Brodie, M.J., Laurenza, A., FitzGibbon, H., and Gidal, B.E., Epilepsy Res., 2015, vol. 117, p. 117.
Yamamoto, Y., Usui, N., Nishida, T., Takahashi, Y., Imai, K., Kagawa, Y., and Inoue, Y., Ther. Drug Monit., 2017, vol. 39, no. 4, p. 446.
Patsalos, P.N., Gougoulaki, M., and Sander, J.W., Ther. Drug Monit., 2016, vol. 38, no. 3, p. 354.
Panday, D.R., Panday, K.R., Basnet, M., Kafle, S., Shah, B., and Rauniar, G.P., Int. J. Neurorehabil., 2017, vol. 4, no. 1, p. 245.
Plosker, G.L., CNS Drugs, 2012, vol. 26, p. 1085.
Mano, Y., Takenaka, O., and Kusano, K., J. Pharm. Biomed. Anal., 2015, vol. 107, p. 56.
Mohamed, S., Candela, C., Riva, R., and Contin, M., Pract. Lab. Med., 2018, vol. 10, p. 15.
Meirinho, S., Rodrigues, M., Fortuna, A., Falcão, A., and Alves, G., J. Pharm. Anal., 2021, vol. 11, no. 4, p. 405.
Franco, V., Marchiselli, R., Fattore, C., Tartara, E., Sarro, G.D., Russo, E., and Perucca, E., Ther. Drug Monit., 2016, vol. 38, no. 6, p. 744.
Patsalos, P.N., Zugman, M., Lake, C., James, A., Ratnaraj, N., and Sander, J.W., Epilepsia, 2017, Vol. 58, no. 7, p. 1234.
Steinhoff, B.J., Hübers, E., Kurth, C., and Jürges, U., Seizure, 2019, vol. 67, p. 18.
British Pharmacopoeia, London: Medicines and Healthcare Products Regulatory Agency (MHRA), 2019, vol. 1.
United States Pharmacopeia, Rockville: United States Pharmacopeial Convention, 2018.
Palmer, L., Bertilsson, L., Collste, P., and Rawlins, M., Clin. Pharmacol. Ther., 1973, vol. 14, no. 5, p. 827.
Messiha, F.S., Alcohol, 1986, vol. 3, p. 135.
Minkova, G. and Getova, D., Methods Find. Exp. Clin. Pharmacol., 2001, vol. 23, no. 9, p. 481.
Dordevic, S., Kilibarda, V., and Stojanovic, T., Vojnosanit. Pregl., 2009, vol. 66, no. 5, p. 347.
Budikayanti, A., Chaliana, C., Louisa, M., and Setiabudy, R., Int. J. Pharm. Sci., 2017, vol. 9, no. 9, p. 87.
Dural, E., Cetin, S., Bolayir, A., and Cigdem, B., J. Faculty Pharm. Istanbul Univ., 2020, vol. 50, no. 1, p. 6.
Grazia, U.D., Durso, A., Ranzato, F., Riva, V.D., Contarato, G., Billo, G., Perini, F., and Galloni, E., Ther. Drug Monit., 2018, vol. 40, no. 4, p. 47.
Rogawski, M.A. and Hanada, T., Acta Neurol. Scand., 2013, vol. 127, p. 19.
Ayscough, N., Fawell, J., Franklin, G., and Young, W., Review of Human Pharmaceuticals in the Environment (P390), Bristol: Environ. Agency, 2000.
Gidal, B.E., Maganti, R., Laurenza, A., Yang, H., Verbal, D.A., Schuck, E., and Ferry, J., Epilepsy Res., 2017, vol. 134, p. 41.
Guideline on Bioanalytical Method Validation, rev. 1, Amsterdam: Eur. Med. Agency, 2015.
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Said A. Hassan, Helmy, A.H., Weshahy, S.A. et al. Development and Bioanalytical Validation of RP-HPLC Method for the Simultaneous Determination of Perampanel and Carbamazepine in Human Plasma for Therapeutic Drug Monitoring. J Anal Chem 78, 622–627 (2023). https://doi.org/10.1134/S1061934823050076
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DOI: https://doi.org/10.1134/S1061934823050076