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Spectrophotometric Determination of Azorubine and Ponceau 4R in Pharmaceutical Preparations Using Micellar Extraction

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Abstract

We compared the capabilities of extraction systems based on polyoxyethylated alkylphenols Triton X-100 and OP-10 for extracting synthetic food dyes Azorubine and Ponceau 4R in different phase separation modes: cloud-point temperature and salting-out with sodium sulfate. The effects of temperature, pH, surfactant, and electrolyte concentrations on the physicochemical characteristics of the micellar phase and the rate for reaching phase equilibrium are studied. A simple and effective extraction procedure with salting-out is proposed. The optimal conditions for the maximum extraction of the dyes (R > 98%) were determined in various modes: at the cloud point for Azorubine and upon salting-out for Ponceau 4R. Procedures have been developed for the spectrophotometric determination of analytes in syrups and lozenges with micellar-extraction preconcentration.

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Authors and Affiliations

  1. Department of Pharmacy, Razumovsky State Medical University, 410012, Saratov, Russia

    N. B. Shestopalova & Yu. A. Fomina

  2. Institute of Chemistry, Saratov State University, 410012, Saratov, Russia

    R. K. Chernova

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  1. N. B. Shestopalova
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  2. Yu. A. Fomina
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  3. R. K. Chernova
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Correspondence to N. B. Shestopalova.

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Translated by O. Zhukova

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Shestopalova, N.B., Fomina, Y.A. & Chernova, R.K. Spectrophotometric Determination of Azorubine and Ponceau 4R in Pharmaceutical Preparations Using Micellar Extraction. J Anal Chem 77, 1073–1081 (2022). https://doi.org/10.1134/S1061934822080135

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