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Microextraction of 17-β-Estradiol from Medicinal Preparations for the Subsequent Determination by HPLC-UV

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Abstract

A method is proposed for the chromatographic determination of 17-β-estradiol in medicinal preparations with the microextraction of the analyte from a sample solution. The procedure involves dispersing the extractant (1-dodecanol) in the aqueous phase by rotating a cotton disk with a magnetic stirrer insert. The formation of the extractant emulsion ensures the rapid mass transfer of the target analyte. When the disk rotates in the aqueous phase, a central vortex also forms, in which a drop of the extract is formed with the subsequent destruction of the emulsion. Cooling the extraction system allows the crystallization of the extract, which is taken for an HPLC-UV analysis. The limit of detection (3σ) of 0.03 mg/L is reached. The analytical capabilities of the developed method are shown on an example of the determination of 17-β-estradiol in transdermal gels.

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Authors and Affiliations

  1. Institute of Chemistry, St. Petersburg State University, 198504, Peterhof, St. Petersburg, Russia

    Z. R. Yakupova, S. A. Lebedinets, K. S. Vakh & A. V. Bulatov

  2. Kazan National Research Technological University, 420015, Kazan, Republic of Tatarstan, Russia

    Z. R. Yakupova & S. Yu. Garmonov

Authors
  1. Z. R. Yakupova
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  2. S. A. Lebedinets
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  3. K. S. Vakh
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  4. S. Yu. Garmonov
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  5. A. V. Bulatov
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Correspondence to Z. R. Yakupova.

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Translated by V. Kudrinskaya

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Yakupova, Z.R., Lebedinets, S.A., Vakh, K.S. et al. Microextraction of 17-β-Estradiol from Medicinal Preparations for the Subsequent Determination by HPLC-UV. J Anal Chem 77, 342–346 (2022). https://doi.org/10.1134/S1061934822010154

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  • DOI: https://doi.org/10.1134/S1061934822010154

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