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A Molecularly Imprinted Polymer as the Adsorbent for the Selective Determination of Oxazepam in Urine and Plasma Samples by High-Performance Liquid Chromatography with Diode Array Detection

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Abstract

Molecularly imprinted polymer for the selective adsorption of oxazepam was synthesized using precipitation polymerization in the presence of oxazepam as a template, ethylene glycol dimethacrylate as a crosslinking agent, methacrylic acid as a functional monomer and 2,2-azobisisobutyronitrile as an initiator. The polymer was characterized by scanning electron microscopy, Fourier-transform infrared spectroscopy and thermogravimetric analysis. The obtained molecular imprinting polymer showed high affinity to template molecule and was used as a selective sorbent for selective extraction of oxazepam from human urine and plasma samples. Good linearity was obtained for the analyte in the range of 2.0–600 µg/L with relative standard deviation ≤ 4.0%. Under optimal conditions, maximum adsorption capacity obtained was 136 mg/g. The detection limit of the proposed procedure was 0.5 µg/L. Finally, this method was applied to the determination of oxazepam in plasma and urine samples and satisfactory results were achieved.

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Funding

Financial support by Lahijan Branch, Islamic Azad University (Lahijan, Iran) during the period of this research is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Chemistry, Lahijan Branch, Islamic Azad University, P.O. Box 4416939515, Lahijan, Iran

    Atefeh Bakhshi, Ameneh Porgham Daryasari & Mojtaba Soleimani

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  1. Atefeh Bakhshi
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  2. Ameneh Porgham Daryasari
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  3. Mojtaba Soleimani
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Correspondence to Ameneh Porgham Daryasari.

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Bakhshi, A., Daryasari, A.P. & Soleimani, M. A Molecularly Imprinted Polymer as the Adsorbent for the Selective Determination of Oxazepam in Urine and Plasma Samples by High-Performance Liquid Chromatography with Diode Array Detection. J Anal Chem 76, 1414–1421 (2021). https://doi.org/10.1134/S1061934821120029

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