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Flow-Injection Lucigenin–Cu(III) Complex Chemiluminescence Determination of Cysteine and Glutathione in Pharmaceutical Formulations

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Abstract

A flow injection method for the determination of cysteine (CySH) and glutathione (GSH) amino acids is reported based on lucigenin−diperiodatocuprate(III) complex chemiluminescence (CL) reaction in basic medium. Under optimum conditions, linear calibration curves were obtained in the range of 0.001–30 (R2 = 0.9998, n = 6) and 0.005–30 mg/L (R2 = 0.9989, n = 6) with detection limits (S/N = 3) of 2.5 × 10–4 and 8 × 10–4 mg/L for CySH and GSH, respectively. The relative standard deviation was 4.1% (n = 11) with injection throughput of 160 per hour. We have applied the developed method to determine CySH and GSH in pharmaceutical samples and compared the results with a spectrophotometric method. Statistical tests such as Student’s t- and F-tests were applied for both results with no significant difference observed at a 95% confidence level. The CL reaction of lucigenin−copper(III) complex is also presented.

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ACKNOWLEDGMENTS

The authors also acknowledge the Department of Chemistry, University of Balochistan, Quetta, Pakistan for providing laboratory facilities.

Funding

One of the authors acknowledges the Higher Education commission, Islamabad for providing laptop and financial support under Prime Minister of Pakistan Laptop and Fee Reimbursement Scheme.

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Authors and Affiliations

  1. Department of Chemistry, University of Balochistan, 87300, Quetta, Pakistan

    Zahoor Ahmed, Mohammad Yaqoob, Muhammad Asghar, Samar Ali & Abdul Nabi

  2. Department of Chemistry, Sardar Bahadur Khan Women’s University, Quetta, Pakistan

    Nusrat Munawar

  3. Department of Botany, University of Balochistan, 87300, Quetta, Pakistan

    Abdul Kabir Khan Achakzai

Authors
  1. Zahoor Ahmed
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  2. Mohammad Yaqoob
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  3. Muhammad Asghar
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  4. Samar Ali
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  5. Nusrat Munawar
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  6. Abdul Kabir Khan Achakzai
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Correspondence to Mohammad Yaqoob.

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Ahmed, Z., Yaqoob, M., Asghar, M. et al. Flow-Injection Lucigenin–Cu(III) Complex Chemiluminescence Determination of Cysteine and Glutathione in Pharmaceutical Formulations. J Anal Chem 76, 466–475 (2021). https://doi.org/10.1134/S1061934821040134

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  • DOI: https://doi.org/10.1134/S1061934821040134

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