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Horseradish Peroxidase-Based Biosensors with Different Nanotransducers for the Determination of Hydrogen Peroxide

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Abstract

We studied methods of the in situ synthesis of gold nanostructures on the electrode surface and of the directed electrochemical synthesis of nanodendrites to create biosensors based on horseradish peroxidase. The effect of nanostructuring of the transducer surface and its subsequent modification on the electroanalytical characteristics of sensors for the determination of hydrogen peroxide was studied. The use of these methods can significantly improve the performance characteristics of the biosensors, such as the sensitivity coefficient, limit of detection, and stability in the range of micromolar and submicromolar concentrations of hydrogen peroxide. The electrodes modified by the in situ synthesis of nanostructures with subsequent treatment with Meerwein’s reagent, as well as ultramicroelectrodes prepared by directional electrochemical synthesis, show low limits of detection for hydrogen peroxide, 0.2 and 0.08 µM, and high sensitivity coefficients, 0.71 and 0.84 A M–1 cm–2, respectively.

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Funding

This work was supported by the Russian Science Foundation, project no. 20-13-00143.

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Authors and Affiliations

  1. ITMO University, 191002, St. Petersburg, Russia

    K. G. Nikolaev

  2. Institute of Chemistry, St. Petersburg State University, 199034, St. Petersburg, Russia

    S. S. Ermakov, Yu. E. Ermolenko & D. V. Navolotskaya

  3. Institute of Biological Information Processing, Forschungszentrum Jülich, 52425, Jülich, Germany

    A. Offenhäusser & Yu. G. Mourzina

Authors
  1. K. G. Nikolaev
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  2. S. S. Ermakov
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  3. Yu. E. Ermolenko
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  4. D. V. Navolotskaya
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  5. A. Offenhäusser
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  6. Yu. G. Mourzina
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Corresponding author

Correspondence to Yu. G. Mourzina.

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Translated by O. Zhukova

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Nikolaev, K.G., Ermakov, S.S., Ermolenko, Y.E. et al. Horseradish Peroxidase-Based Biosensors with Different Nanotransducers for the Determination of Hydrogen Peroxide. J Anal Chem 76, 510–517 (2021). https://doi.org/10.1134/S1061934821040080

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  • DOI: https://doi.org/10.1134/S1061934821040080

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