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Ratios of Stable Isotopes 13С/12С and 15N/14N in Samples of Dead Honey Bees and Beekeeping Products

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Abstract

The isotopic composition of carbon (δ13С) and nitrogen (δ15N) in samples of dead honey bees and beekeeping products (honey, pollen load, wax, propolis) purchased both at honey fairs and directly at apiaries in the Tomsk oblast and, for comparison, in the Altai krai, Krasnodar krai, and Kaluga and Kemerovo oblasts, was analyzed. The goal of the study was to identify the indicator ability of the ratios of stable isotopes of carbon 13C/12C and nitrogen 15N/14N in monitoring the ecological state of territories covering the habitat of honey bees and controlling food products (honey, pollen load). Isotope analysis was performed by isotope mass spectrometry using a DELTA V Advantage isotope mass spectrometer (Thermo Fisher Scientific, Germany).

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REFERENCES

  1. Lebedev, A.T., Mass-spektrometriya dlya analiza ob”ektov okruzhayushchei sredy (Mass Spectrometry for the Analysis of Environmental Samples), Moscow: Tekhnosfera, 2013.

  2. Kadirov, R.A., Cand. Sci. (Biol.) Dissertation, Moscow, 1999.

  3. Korkina, V.I., Sib. Vestn. Sel’skokhoz. Nauki, 2008, no. 5, p. 154.

  4. Petukhov, A.V., Ulanova, T.S., and Zavgorodnyaya, I.S., in Voprosy apidologii: Sbornik statei (Apidology Issues: Collection of Papers), Izhevsk: Udmurt. Gos. Nauchno-Issled. Inst. Sel’sk. Khoz., 2000, p. 76.

  5. Taki, H., Ikeda, H., Nagamitsu, T., Yasuda, M., Sugiura, S., Maeto, K., and Okabe, K., Biodiversity Conserv., 2017, vol. 26, p. 3157.

    Article  Google Scholar 

  6. AOAC Official Methods of Analysis, Method 998.12: C-4 Plant Sugars in Honey. Internal Standard Stable Carbon Isotope Ratio Method, AOAC International, 1998.

    Google Scholar 

  7. Cerling, T.E., Barnette, J.E., Bowen, G.J., Chesson, L.A., Ehleringer, J.R., Remien, C.H., Shea, P., Tipple, B.J., and West, J.B., Annu. Rev. Earth Planet. Sci., 2016, vol. 44, p. 175.

    Article  CAS  Google Scholar 

  8. Xiaoteng, Z., Taylor, M.P., Salouros, H., and Prasad, S., Sci. Rep., 2018, vol. 8, 14639.

    Article  Google Scholar 

  9. White, J.W. and Winters, K., J. Assoc. Off. Anal. Chem., 1989, vol. 72, p. 907.

    CAS  PubMed  Google Scholar 

  10. Vetrova, O.V., Kalashnikova, D.A., Melkov, V.N., and Simonova, G.V., J. Anal. Chem., 2017, vol. 72, no. 7, p. 756.

    Article  CAS  Google Scholar 

  11. Rankovic, A., Geslinc, B., Perrard, A., Barbillon, A., Vaury, V., Abbadie, L., and Dajoz, I., Acta Oecol., 2020, vol. 105, 103545.

    Article  Google Scholar 

  12. Galimov, E.M., Priroda biologicheskogo fraktsionirovaniya izotopov (The Nature of Biological Isotope Fractionation), Moscow: Nauka, 1981.

  13. Padovan, G.J., Rodriques, L.P., Leme, I.A., Jong, D.D., and Marchini, J.S., Eurasian J. Anal. Chem., 2007, vol. 2, no. 3, p. 134.

    CAS  Google Scholar 

  14. Jonasson, S., Medrano, H., and Flexas, J., Funct. Ecol., 1997, vol. 11, no. 3, p. 282.

    Article  Google Scholar 

  15. Veiga, S.J., MS Thesis, Botucatu, Brazil: Univ. Estadual Paulista, 2017.

  16. Bontempo, L., Camin, F., Ziller, L., Perini, M., Nicolini, G., and Larcher, R., Measurement, 2017, vol. 98, p. 283.

    Article  Google Scholar 

  17. Kropf, U., Golob, T., Necemer, M., Kump, P., Korosec, M., Bertoncelj, J., and Ogrinc, N., J. Agric. Food Chem., 2010, vol. 58, p. 12794.

    Article  CAS  Google Scholar 

  18. Rogers, K.M., Rapid Commun. Mass Spectrom., 2010, vol. 24, p. 2370.

    Article  CAS  Google Scholar 

  19. Pascoal, A., Rodrigues, S., Teixeira, A., Feás, X., and Estevinho, L.M., Food Chem. Toxicol., 2014, vol. 63, p. 233.

    Article  CAS  Google Scholar 

  20. Raschi, A., Miglietta, F., Tognetti, R., and Gardingen, P.V., Plant Responses to Elevated CO 2 : Evidence from Natural Springs, Cambridge: Cambridge Univ. Press, 1997.

    Book  Google Scholar 

  21. Merwe, N.V.D. and Medina, E.T.G., J. Archeol. Sci., 1991, vol. 18, p. 249.

    Article  Google Scholar 

  22. Bengsch, E., Kettrup, A., Stocker, A., and Rossmann, A., FRG Patent DE10344606, 2011.

  23. How do bees make honeycombs and what are they for? https://ferma.expert/pchely/pcheloprodukciya/pchelinye-soty. Accessed August 25, 2020.

  24. Abelson, P.H. and Hoering, T.C., Proc. Natl. Acad. Sci. U. S. A., 1961, vol. 47, no. 5, p. 623.

    Article  CAS  Google Scholar 

  25. Kodina, L.A. and Generalova, V.N., Referaty dokladov i soobshchenii IX Mendeleevskogo s”ezda po obshchei i prikladnoi khimii, Alma-Ata (Proc. IX Mendeleev Congress on General and Applied Chemistry, Alma-Ata), Moscow: Nauka, 1975, vol. 1, p. 308.

  26. Kodina, L.A., Geochem. Int., 2010, vol. 48, no. 12, p. 1157.

    Article  Google Scholar 

  27. Hyodo, F., Entomol. Sci., 2015, vol. 18, p. 295.

    Article  Google Scholar 

  28. Schellenberg, A., Chmielus, S., Schlicht, C., Camin, F., Perini, M., Bontempo, L., Heinrich, K., Kelly, S.D., Rossmann, A., Thomas, F., Jamin, E., and Horacek, M., Food Chem., 2010, vol. 121, p. 770.

    Article  CAS  Google Scholar 

  29. Bateman, A.S. and Kelly, S.D., Isotopes Environ. Health Stud, 2007, vol. 43, no. 3, p. 237.

    Article  CAS  Google Scholar 

  30. Makarov, M.I., Eurasian Soil Sci., 2009, vol. 42, p. 1335.

    Article  Google Scholar 

  31. Szpak, P., Front. Plant Sci., 2014, vol. 5, p. 288.

    Article  Google Scholar 

  32. Nommik, H., Pluth, D.J., Larsson, K., and Mahendrappa, M.K., Plant Soil, 1994, vol. 158, p. 169.

    Article  CAS  Google Scholar 

  33. Stocker, A., Rossmann, A., Kettrup, A., and Bengsch, E., Rapid Commun. Mass Spectrom., 2006, vol. 20, no. 2, p. 181.

    Article  CAS  Google Scholar 

  34. Schwarz, D.M., A stable isotope investigation of pollen from Pinery Provincial Park, Southwestern Ontario, Canada, Thesis, Electronic Thesis and Dissertation Repository, 2016, no. 4254.

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Funding

The study was supported by the Russian Foundation for Basic Research, project no. 19-34-90016.

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Authors and Affiliations

  1. Institute of Monitoring of Climatic and Ecological Systems, Siberian Branch, Russian Academy of Sciences, 634055, Tomsk, Russia

    D. A. Kalashnikova & G. V. Simonova

Authors
  1. D. A. Kalashnikova
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  2. G. V. Simonova
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Correspondence to D. A. Kalashnikova.

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Translated by O. Zhukova

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Kalashnikova, D.A., Simonova, G.V. Ratios of Stable Isotopes 13С/12С and 15N/14N in Samples of Dead Honey Bees and Beekeeping Products. J Anal Chem 76, 526–534 (2021). https://doi.org/10.1134/S1061934821040067

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  • DOI: https://doi.org/10.1134/S1061934821040067

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