Abstract
A convenient, cheap and accurate method for the determination of quisqualic acid in traditional Chinese medicine Quisqualis fructus by pre-column derivatization with 2-nitrobenzenesulfonyl chloride and HPLC was established. Quisqualis fructus samples were homogenised in water after being crushed and degreased with petroleum ether. Target compounds were separated and detected on a C18 column using gradient elution. The recovery rates were in the range of 90–103%. Good linearity within the range of 0.2–20 mg/mL for quisqualic acid with correlation coefficients of ≥0.98 was observed. The relative standard deviations (RSDs) of the within-day and between-day retention time precisions were 0.15–0.17% and 0.8–0.9%, respectively. The RSDs of the within-day and between-day integrated area precisions were 2.3–6.4% and 4.8–9.8%, respectively. The concentrations of quisqualic acid (1.5 mg/g) from Quisqualis fructus were conveniently measured without any expensive photodiode array detector but with a common UV–visible detector.
Similar content being viewed by others
REFERENCES
Fang, S.D. and Zhu, R.H., Acta Chim. Sin., 1964, vol. 30, p. 226.
Boden, P., Bycroft, B.W., Chhabra, S.R., Chiplin, J., Crowley, P.J., Grout, R.J., King, T.J., Mcdonald, E., Rafferty, P., and Usherwood, P.N.R., Brain. Res., 1986, vol. 385, p. 205.
Monaghan, D.T., Bridges, R.J., and Cotman, C.W., Annu. Rev. Pharmacol., 2003, vol. 29, p. 365.
Rochford, J., Sen, A.P., Rousse, I., and Welner, S.A., Brain Res., 1996, vol. 41, p. 313.
Bitzer, M. and Schaeffel, F., Optom. Visual Sci., 2004, vol. 81, p. 127.
Kimura, S., Kawasaki, S., Takashima, K., and Sasaki, K., Jpn. J. Physiol., 2001, vol. 51, p. 511.
Yoshiyama, H., Jpn. Patent JPS51127080, 1976.
Baldwin, J.E., Adlington, R.M., Birch, D.J., Crawford, J.A., and Sweeney, J.B., Cheminformatics, 1985, vol. 18, p. 1339.
Murakoshi, I., Kaneko, M., Koide, C., and Ikegami, F., Phytochemistry, 1986, vol. 25, p. 2759.
Pharmacopoeia of the People’s Republic of China, Beijing, 2010, vol. 1, p. 392.
Ranger, C.M., Winter, R.E., Singh, A.P., Reding, M.E., Frantz, J.M., Locke, J.C., and Krause, C.R.P., Natl. Acad. Sci. U. S. A., 2011, vol. 108, p. 1217.
Vendrell, J. and Aviles, F.X., J. Chromatogr. A, 1986, vol. 358, p. 401.
Wang, C.Y., Sun, Y., Wen, Q.Y., Wang, G.X., Yang, W.X.S., Qu, Q., Yang, G.J., and Hu, X.Y., Talanta, 2010, vol. 81, p. 1613.
Zhang, X.L., Zhao, T., Cheng, T., Liu, X., and Zhang, H.X., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 2012, vol. 906, p. 91.
Funding
This work was supported by the National Natural Science Foundation of China (21376119, 81673321, 21506099), and Specialized Research Fund for the Doctoral Program of Higher Education (20123221130001).
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
It is confirmed that there is no conflict of interest.
Rights and permissions
About this article
Cite this article
Chang, S., Bingfeng Li, Chen, T. et al. Determination of Quisqualic Acid in quisqualis fructus by Pre-column Derivatization and High Performance Liquid Chromatography. J Anal Chem 75, 1018–1023 (2020). https://doi.org/10.1134/S1061934820080055
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1061934820080055