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Determination of Quisqualic Acid in quisqualis fructus by Pre-column Derivatization and High Performance Liquid Chromatography

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Abstract

A convenient, cheap and accurate method for the determination of quisqualic acid in traditional Chinese medicine Quisqualis fructus by pre-column derivatization with 2-nitrobenzenesulfonyl chloride and HPLC was established. Quisqualis fructus samples were homogenised in water after being crushed and degreased with petroleum ether. Target compounds were separated and detected on a C18 column using gradient elution. The recovery rates were in the range of 90–103%. Good linearity within the range of 0.2–20 mg/mL for quisqualic acid with correlation coefficients of ≥0.98 was observed. The relative standard deviations (RSDs) of the within-day and between-day retention time precisions were 0.15–0.17% and 0.8–0.9%, respectively. The RSDs of the within-day and between-day integrated area precisions were 2.3–6.4% and 4.8–9.8%, respectively. The concentrations of quisqualic acid (1.5 mg/g) from Quisqualis fructus were conveniently measured without any expensive photodiode array detector but with a common UV–visible detector.

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REFERENCES

  1. Fang, S.D. and Zhu, R.H., Acta Chim. Sin., 1964, vol. 30, p. 226.

    CAS  Google Scholar 

  2. Boden, P., Bycroft, B.W., Chhabra, S.R., Chiplin, J., Crowley, P.J., Grout, R.J., King, T.J., Mcdonald, E., Rafferty, P., and Usherwood, P.N.R., Brain. Res., 1986, vol. 385, p. 205.

    Article  CAS  Google Scholar 

  3. Monaghan, D.T., Bridges, R.J., and Cotman, C.W., Annu. Rev. Pharmacol., 2003, vol. 29, p. 365.

    Article  Google Scholar 

  4. Rochford, J., Sen, A.P., Rousse, I., and Welner, S.A., Brain Res., 1996, vol. 41, p. 313.

    Article  CAS  Google Scholar 

  5. Bitzer, M. and Schaeffel, F., Optom. Visual Sci., 2004, vol. 81, p. 127.

    Article  Google Scholar 

  6. Kimura, S., Kawasaki, S., Takashima, K., and Sasaki, K., Jpn. J. Physiol., 2001, vol. 51, p. 511.

    Article  CAS  Google Scholar 

  7. Yoshiyama, H., Jpn. Patent JPS51127080, 1976.

  8. Baldwin, J.E., Adlington, R.M., Birch, D.J., Crawford, J.A., and Sweeney, J.B., Cheminformatics, 1985, vol. 18, p. 1339.

    Google Scholar 

  9. Murakoshi, I., Kaneko, M., Koide, C., and Ikegami, F., Phytochemistry, 1986, vol. 25, p. 2759.

    Article  CAS  Google Scholar 

  10. Pharmacopoeia of the People’s Republic of China, Beijing, 2010, vol. 1, p. 392.

  11. Ranger, C.M., Winter, R.E., Singh, A.P., Reding, M.E., Frantz, J.M., Locke, J.C., and Krause, C.R.P., Natl. Acad. Sci. U. S. A., 2011, vol. 108, p. 1217.

    Article  CAS  Google Scholar 

  12. Vendrell, J. and Aviles, F.X., J. Chromatogr. A, 1986, vol. 358, p. 401.

    Article  CAS  Google Scholar 

  13. Wang, C.Y., Sun, Y., Wen, Q.Y., Wang, G.X., Yang, W.X.S., Qu, Q., Yang, G.J., and Hu, X.Y., Talanta, 2010, vol. 81, p. 1613.

    Article  CAS  Google Scholar 

  14. Zhang, X.L., Zhao, T., Cheng, T., Liu, X., and Zhang, H.X., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 2012, vol. 906, p. 91.

    Article  CAS  Google Scholar 

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Funding

This work was supported by the National Natural Science Foundation of China (21376119, 81673321, 21506099), and Specialized Research Fund for the Doctoral Program of Higher Education (20123221130001).

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Correspondence to Siyuan Chang or Xuejun He.

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Chang, S., Bingfeng Li, Chen, T. et al. Determination of Quisqualic Acid in quisqualis fructus by Pre-column Derivatization and High Performance Liquid Chromatography. J Anal Chem 75, 1018–1023 (2020). https://doi.org/10.1134/S1061934820080055

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  • DOI: https://doi.org/10.1134/S1061934820080055

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