Journal of Analytical Chemistry

, Volume 74, Issue 5, pp 467–471 | Cite as

Development and Validation of Analytical Method for Simultaneous Estimation of Tazarotene and Hydroquinone in their Gel Formulation by Reversed-phase High Performance Liquid Chromatography

  • Yadav Rajkumar
  • Yadav Divya
  • Yadav RakeshEmail author


A simple, sensitive, rapid, precise and accurate method has been developed for simultaneous estimation of tazarotene and hydroquinone in their pharmaceutical dosage form (gel preparation). The chromatographic separation was carried out on a reversed-phase Inertsil C18 (4.6 mm I.D. × 250 mm, 5 μm) column using a mobile phase consisting of 0.02 M KH2PO4‒acetonitrile (80 : 20, v/v) at a flow rate of 1.0 mL/min and UV detection at λmax = 254 nm. The method showed linearity with the correlation coefficient of 0.999 for both tazarotene and hydroquinone over the concentration range of 5‒25 and 1‒5 µg/mL, respectively. The mean recoveries were found to be in the range of 98‒101% for both components. The method was validated as per International Conference on Harmonization guidelines for linearity, limit of detection, limit of quantification, specificity, accuracy, precision and robustness. As a result, this method can be successfully applied for routine quantification of tazarotene and hydroquinone in pharmaceutical dosage form (gel preparation).


tazarotene hydroquinone simultaneous estimation RP-HPLC 



Authors gratefully acknowledge Vice-chancellor, Banasthali Vidyapith for providing necessary research facilities. The financial assistance provided by the DST, New Delhi under the CURIE program is duly acknowledged.


  1. 1.
    Brenna, E., Frigoli, S., Fronza, G., and Serra, S., J. Pharm. Biomed. Anal., 2008, vol. 46, no. 3, p. 574.CrossRefGoogle Scholar
  2. 2.
    Tazarotene on php?searchterm=tazarotene. Accessed December 18, 2016.Google Scholar
  3. 3.
    Sheliya, K., Shah, K., and Kapupara, P., J. Chem. Pharm. Res., 2014, vol. 6, no. 4, p. 934.Google Scholar
  4. 4.
    Odumosu, P. and Ekwe, T., Afr. J. Pharm. Pharmacol., 2010, vol. 4, no. 5, p. 231.Google Scholar
  5. 5.
    Desiderio, C., Ossicini, L., and Fanali, S., J. Chromatogr. A, 2000, vol. 887, p. 489.CrossRefGoogle Scholar
  6. 6.
    Lowe, N., Horwitz, S., Tanghetti, E., Draelos, Z., and Menter, A., J. Cosmet. Laser Ther., 2006, vol. 8, no. 3, p. 121.CrossRefGoogle Scholar
  7. 7.
    Elzanfaly, E., Saad, A., and Elaleem, A., Saudi Pharm. J., 2012, vol. 20, p. 249.CrossRefGoogle Scholar
  8. 8.
    Jogarami, R., Jain, P., and Sharma, S., J. Pharm. Res., 2012, vol. 5, p. 2273.Google Scholar
  9. 9.
    Patel, M., Patel, R., Parikh, J., and Patel, B., Anal. Methods, 2010, vol. 2, p. 275.CrossRefGoogle Scholar
  10. 10.
    Pathare, D., Jadhav, S., and Shingare, M., Chromatographia, 2007, vol. 66, p. 247.CrossRefGoogle Scholar
  11. 11.
    Badawy, A.M., El-Alim, A.A., and Saad, A.S., Drug Test. Anal., 2010, vol. 2, p. 130.Google Scholar
  12. 12.
    Roy, C. and Chakrabarty, J., J. Sci. Pharm., 2013, vol. 81, no. 4, p. 951.Google Scholar
  13. 13.
    Roy, C., Patel, H., and Chakrabarty, J., Indo Am. J. Pharm. Sci., 2012, vol. 3, p. 1400.Google Scholar
  14. 14.
    International Conference on Harmonization (ICH) Guidelines Topic Q2(R1): Validation of Analytical Procedures: Text and Methodology, Geneva, 2005. http://www. Guidelines/Quality/Q2_R1/Step4/Q2_R1__Guideline.pdf. Accessed October 10, 2016.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  1. 1.Department of Pharmacy, Banasthali VidyapithRajasthanIndia

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