Journal of Analytical Chemistry

, Volume 74, Issue 2, pp 143–152 | Cite as

Reducing and Oxidizing Columns in the Flow Injection Determination of Nitrazepam in Pharmaceutical Formulations

  • Hind HadiEmail author
  • Marwa Mouayed
  • Ayman A. Gouda


Two flow injection analysis (FIA) systems for determining nitrazepam (NZP) in pharmaceutical formulations using two kinds of solid-phase reactors were developed. The first system involved on-line oxidation of the reagent using a mini column containing PbO2 immobilized on cellulose acetate, while the second one involved on-line reduction of drug using a mini-Jones reductor. Both methods are based on spectrophotometric method involving oxidative coupling reaction to phenothiazine with reduced NZP in the presence of a suitable oxidizing agent to form a green color product measured at 615 or 589 nm for the two methods, respectively. The calibration graphs were linear over the range 0.5 to 45 and 10 to 250 μg/mL with a relative standard deviation less than 3.6% (n = 32) and 2.4% (n = 35) for the two methods. Solid-phase reactors can be applied for the determination of NZP in a FIA with high sensitivity and significant advantages over conventional procedures.


solid phase reactors nitrazepam reverse flow injection 


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Copyright information

© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  1. 1.Department of Chemistry, College of Science, University of BaghdadBaghdadIraq
  2. 2.Chemistry Department, Faculty of Sciences, Zagazig UniversityZagazigEgypt

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