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Reducing and Oxidizing Columns in the Flow Injection Determination of Nitrazepam in Pharmaceutical Formulations

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Abstract

Two flow injection analysis (FIA) systems for determining nitrazepam (NZP) in pharmaceutical formulations using two kinds of solid-phase reactors were developed. The first system involved on-line oxidation of the reagent using a mini column containing PbO2 immobilized on cellulose acetate, while the second one involved on-line reduction of drug using a mini-Jones reductor. Both methods are based on spectrophotometric method involving oxidative coupling reaction to phenothiazine with reduced NZP in the presence of a suitable oxidizing agent to form a green color product measured at 615 or 589 nm for the two methods, respectively. The calibration graphs were linear over the range 0.5 to 45 and 10 to 250 μg/mL with a relative standard deviation less than 3.6% (n = 32) and 2.4% (n = 35) for the two methods. Solid-phase reactors can be applied for the determination of NZP in a FIA with high sensitivity and significant advantages over conventional procedures.

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Authors and Affiliations

  1. Department of Chemistry, College of Science, University of Baghdad, Baghdad, Iraq

    Hind Hadi & Marwa Mouayed

  2. Chemistry Department, Faculty of Sciences, Zagazig University, 44519, Zagazig, Egypt

    Ayman A. Gouda

Authors
  1. Hind Hadi
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  2. Marwa Mouayed
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  3. Ayman A. Gouda
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Correspondence to Hind Hadi.

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Hind Hadi, Mouayed, M. & Gouda, A.A. Reducing and Oxidizing Columns in the Flow Injection Determination of Nitrazepam in Pharmaceutical Formulations. J Anal Chem 74, 143–152 (2019). https://doi.org/10.1134/S1061934819020060

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  • DOI: https://doi.org/10.1134/S1061934819020060

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