Journal of Analytical Chemistry

, Volume 73, Issue 4, pp 358–363 | Cite as

Simultaneous Determination of Succinic Acid and Water-Soluble Vitamins by Ion-Pair High-Performance Liquid Chromatography

  • K. A. LeonovEmail author
  • A. V. Pustovoitov
  • D. A. Vishenkova


A procedure is proposed for the determination of succinic acid, riboxin, nicotinamide, and riboflavin by ion-pair HPLC with UV detection. Owing to the addition of an ion-pair modifier to the mobile phase and the selection of gradient elution conditions, the optimal retention and resolution of peaks of the components to be determined are achieved. Specificity, linearity, accuracy, and precision of the developed procedure are proved on an example of the determination of active substances in the Cerebronorm® preparation.


ion-pair HPLC gradient elution succinic acid water-soluble vitamins Cerebronorm® 


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  1. 1.
    Denisova, L.V., Cand. Sci. (Chem.) Dissertation, Moscow: Mendeleev Univ. Chem. Technol., 2004.Google Scholar
  2. 2.
    Bendryshev, A.A., Pashkova, E.B., Pirogov, A.V., and Shpigun, O.A., Moscow Univ. Chem. Bull. (Engl. Transl.), 2010, vol. 65, no. 4, p.260.CrossRefGoogle Scholar
  3. 3.
    Shelemet’eva, O.V., Cand. Sci. (Chem.) Dissertation, Tomsk: Tomsk Polytech. Univ., 2009.Google Scholar
  4. 4.
    Heudi, O., Kilinc, T., Fontannaz, P., and Marley, E., J. Chromatogr. A, 2005, vol. 1070, p.49.CrossRefGoogle Scholar
  5. 5.
    Moreno, P. and Salvado, V., J. Chromatogr. A, 2000, vol. 870, p.207.CrossRefGoogle Scholar
  6. 6.
    Marszaii, M.L., Lebiedzinska, A., Czarnowski, W., and Szefer, P., J. Chromatogr. A, 2005, vol. 1094, p.91.CrossRefGoogle Scholar
  7. 7.
    Amin, M. and Reusch, J., Analyst, 1987, vol. 112, p.989.CrossRefGoogle Scholar
  8. 8.
    Woollard, D.C. and Indyk, H.E., J. AOAC Int., 2002, vol. 85, p.945.Google Scholar
  9. 9.
    Li, K., Biomed. Chromatogr., 2002, vol. 16, p.504.CrossRefGoogle Scholar
  10. 10.
    GOST (State Standard) R ISO 5725-1-2002: Accuracy (Trueness and Precision) of Measurement Methods and Results. Part 1. General Principles and Definitions, Moscow: Izd. Standartov, 2002.Google Scholar
  11. 11.
    Guidance for Industry Q2B Validation of Analytical Procedures: Methodology. U.S. Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research (CDER), Center for Biologics Evaluation and Research (CBER), 1996.Google Scholar
  12. 12.
    Barysheva, S.V., Cand. Sci. (Chem.) Dissertation, Saratov: Saratov State Univ., 2003.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • K. A. Leonov
    • 1
    Email author
  • A. V. Pustovoitov
    • 2
  • D. A. Vishenkova
    • 1
  1. 1.Institute of Natural ResourcesNational Research Tomsk Polytechnic UniversityTomskRussia
  2. 2.OOO “Sibtest,”TomskRussia

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