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Journal of Analytical Chemistry

, Volume 72, Issue 13, pp 1280–1288 | Cite as

A “Low-Molecular” Approach to the Identification of Microorganisms by MALDI Mass Spectrometry

  • B. L. MilmanEmail author
  • Yu. Yu. Ilyasov
  • N. V. Lugovkina
  • A. A. Golovina
  • A. V. Dmitriev
Articles

Abstract

A new approach to the identification of microorganisms is presented. It includes the transformation of their MALDI mass spectra aimed at reducing mass scale by one order of magnitude and the use of standard software for building mass spectral libraries of low-molecular compounds and library searches. A library of 728 transformed (“rescaled”) mass spectra of 182 strains for some Streptococcus species was built. With this library, the rate of true microorganism identification was estimated by cross-comparison between library mass spectra (internal validation of the approach). The true identification rate was 84% for three Streptococcus species, which corresponds to the average trueness of species identification by MALDI as found in the literature. The proposed approach to identification can be considered as a method of choice for solving identification problems under consideration.

Keywords

mass spectrometry MALDI identification bacteria mass spectral libraries 

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References

  1. 1.
    Fenselau, C. and Demirev, P.A., Mass Spectrom. Rev., 2001, vol. 20, no. 4, p. 157.CrossRefGoogle Scholar
  2. 2.
    Sauer, S., Freiwald, A., Maier, T., et al., PloS One, 2008, vol. 3, no. 7, e2843.CrossRefGoogle Scholar
  3. 3.
    Sandrin, T.R., Goldstein, J.E., and Schumaker, S., Mass Spectrom. Rev., 2013, vol. 32, no. 3, p. 188.CrossRefGoogle Scholar
  4. 4.
    Kim, T.S., Lee, K., Hong, Y.J., et al., Lab. Med. Online, 2015, vol. 5, no. 4, p. 176.CrossRefGoogle Scholar
  5. 5.
    MALDI Biotyper User Manual, Version 2.0 SR1, Bruker Daltonics, Germany, 2008.Google Scholar
  6. 6.
    VITEK MS Selection of Publications, BioMérieux, 2013. http://www.biomerieux-diagnostics.comsites/clinic/files/selection-of-publications-vitek-ms-final-03-04-2013_0.pdf. Accessed May 23, 2016.Google Scholar
  7. 7.
    Ferroni, A., Suarez, S., Beretti, J.L., et al., J. Clin. Microbiol., 2010, vol. 48, no. 5, p. 1542.CrossRefGoogle Scholar
  8. 8.
    Bille, E., Dauphin, B., Leto, J., et al., Clin. Microbiol. Infect., 2012, vol. 18, no. 11, p. 1117.CrossRefGoogle Scholar
  9. 9.
    Cherkaoui, A., Hibbs, J., Emonet, S., et al., J. Clin. Microbiol., 2010, vol. 48, no. 4, p. 1169.CrossRefGoogle Scholar
  10. 10.
    Seng, P., Drancourt, M., Gouriet, F., et al., Clin. Infect. Dis., 2009, vol. 49, no. 4, p. 543.CrossRefGoogle Scholar
  11. 11.
    Mellmann, A., Bimet, F., Bizet, C., et al., J. Clin. Microbiol., 2009, vol. 47, no. 11, p. 3732.CrossRefGoogle Scholar
  12. 12.
    Stevenson, L.G., Drake, S.K., and Murray, P.R., J. Clin. Microbiol., 2010, vol. 48, no. 2, p. 444.CrossRefGoogle Scholar
  13. 13.
    Van Veen, S.Q., Claas, E.C.J., and Kuijper, E.J., J. Clin. Microbiol., 2010, vol. 48, no. 3, p. 900.CrossRefGoogle Scholar
  14. 14.
    Risch, M., Radjenovic, D., Han, J.N., et al., Swiss Med Wkly, 2010, vol. 140, w13095.Google Scholar
  15. 15.
    Saffert, R.T., Cunningham, S.A., Ihde, S.M., et al., J. Clin. Microbiol., 2011, vol. 49, no. 3, p. 887.CrossRefGoogle Scholar
  16. 16.
    Neville, S.A., LeCordier, A., Ziochos, H., et al., J. Clin. Microbiol., 2011, vol. 49, no. 8, p. 2980.CrossRefGoogle Scholar
  17. 17.
    Cherkaoui, A., Emonet, S., Fernandez, J., et al., J. Clin. Microbiol., 2011, vol. 49, no. 8, p. 3004.CrossRefGoogle Scholar
  18. 18.
    Kaleta, E.J., Clark, A.E., Cherkaoui, A., et al., Clin. Chem., 2011, vol. 57, no. 7, p. 1057.CrossRefGoogle Scholar
  19. 19.
    Carbonnelle, E., Grohs, P., Jacquier, H., et al., J. Microbiol. Methods, 2012, vol. 89, no. 2, p. 133.CrossRefGoogle Scholar
  20. 20.
    Martiny, D., Busson, L., Wybo, I., et al., J. Clin. Microbiol., 2012, vol. 50, no. 4, p. 1313.CrossRefGoogle Scholar
  21. 21.
    Harris, P., Winney, I., Ashhurst-Smith, C., et al., Pathology, 2012, vol. 44, no. 6, p. 583.CrossRefGoogle Scholar
  22. 22.
    Schulthess, B., Bloemberg, G.V., Zbinden, R., et al., J. Clin. Microbiol., 2014, vol. 52, no. 4, p. 1089.CrossRefGoogle Scholar
  23. 23.
    Deak, E., Charlton, C.L., Bobenchik, A.M., et al., Diagn. Microbiol. Infect. Dis., 2015, vol. 81, no. 1, p. 27.CrossRefGoogle Scholar
  24. 24.
    Lévesque, S., Dufresne, P.J., Soualhine, H., et al., PloS One, 2015, vol. 10, no. 12, p. e0144878.CrossRefGoogle Scholar
  25. 25.
    NIST MS Search Program. http://chemdata.nist.gov/mass-spc/ms-search. Accessed May 23, 2016.Google Scholar
  26. 26.
    Mil’man, B.L., Vvedenie v khimicheskuyu identifikatsiyu (Introduction to Chemical Identification), St. Petersburg: VVM, 2008.Google Scholar
  27. 27.
    Milman, B.L., Chemical Identification and Its Quality Assurance, Berlin: Springer, 2011.CrossRefGoogle Scholar
  28. 28.
    Hughes, J.M., Wilson, M.E., Brandt, C.M., and Spellerberg, B., Clin. Infect. Dis., 2009, vol. 49, no. 5, p. 766.CrossRefGoogle Scholar
  29. 29.
    Ikebe, T., Oguro, Y., Ogata, K., et al., Jpn. J. Infect. Dis., 2010, vol. 63, no. 5, p. 372.Google Scholar
  30. 30.
    Briko, N.I., Glushkova, E.V., Nosik, A.G., et al., Zh. Microbiol. Epidemiol. Immunobiol., 2014, no. 5, p. 24.Google Scholar
  31. 31.
    Mayanskiy, N., Alyabieva, N., Ponomarenko, O., et al., Pediatr. Infect. Dis. J., 2015, vol. 34, no. 3, p. 255.CrossRefGoogle Scholar
  32. 32.
    Yatsyshina, S., Mayanskiy, N., Shipulina, O., et al., Diagn. Microbiol. Infect. Dis., 2016, vol. 85, no. 1, p. 125.CrossRefGoogle Scholar
  33. 33.
    Woo, P.C., Fung, A.M., Lau, S.K., et al., J. Clin. Microbiol., 2001, vol. 39, no. 9, p. 3147.CrossRefGoogle Scholar
  34. 34.
    Ilyasov, Yu.Yu., Biswas, I., Totolyan, A.A., and Dmitriev, A.V., Klin. Lab. Diagn., 2011, no. 2, p. 40.Google Scholar
  35. 35.
    Lancefield Classification of Streptococci. http://humbio. ru/humbio/infect_har/00231dfd.htm. Accessed May 23, 2016.Google Scholar
  36. 36.
    Moura, H., Woolfitt, A.R., Carvalho, M.G., et al., FEMS Immunol. Med. Microbiol., 2008, vol. 53, no. 3, p. 333–342.CrossRefGoogle Scholar
  37. 37.
    Wang, J., Zhou, N., Xu, B., et al., PloS One, 2012, vol. 7, no. 11, p. e47152.CrossRefGoogle Scholar
  38. 38.
    Milman, B.L. and Zhurkovich, I.K., Anal. Chem. Res., 2014, vol. 1, p. 8.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  • B. L. Milman
    • 1
    Email author
  • Yu. Yu. Ilyasov
    • 1
  • N. V. Lugovkina
    • 1
    • 2
  • A. A. Golovina
    • 1
  • A. V. Dmitriev
    • 1
  1. 1.Institute of Experimental MedicineSt. PetersburgRussia
  2. 2.Institute of ToxicologySt. PetersburgRussia

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