Abstract
A test method is developed for determining benzo[a]pyrene in natural water, based on the use of a polyethylene filter (frit) with adsorbed specific antibodies, placed within a transparent column. In passing a test solution, the analyte is adsorbed on the frit similarly to the process implemented in immunoaffinity preconcentration. The added conjugate of a labeled analyte takes the remained vacant binding sites of antibodies. Luminescent semiconductor nanoparticles (quantum dots) CdSe/ZnS, used as labels, enable visual determination under irradiation with UV light. The limit of detection for benzo[a]pyrene in water is ~0.5 ng/mL.
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Original Russian Text © N.V. Beloglazova, P.S. Shmelin, A.S. Novikova, I.Yu. Goryacheva, 2017, published in Zhurnal Analiticheskoi Khimii, 2017, Vol. 72, No. 6, pp. 510–514.
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Beloglazova, N.V., Shmelin, P.S., Novikova, A.S. et al. A luminescence immunoassay test method for determining benzo[a]pyrene in natural water. J Anal Chem 72, 597–601 (2017). https://doi.org/10.1134/S106193481706003X
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DOI: https://doi.org/10.1134/S106193481706003X