Journal of Analytical Chemistry

, Volume 72, Issue 5, pp 562–566 | Cite as

Determination of flunixin by sensitized terbium fluorescence in the presence of surfactant micelles

  • T. D. Smirnova
  • S. N. Shtykov
  • E. A. Zhelobitskaya
  • M. I. Safarova


The effect of surfactant micelles and the second ligand on the efficiency of energy transfer in the Tb3+–flunixin complex was studied. The fluorescence intensity of the Tb3+–flunixin complex was shown to 100-fold increase in the presence of Tween 80 micelles and the second trioctylphosphine oxide ligand. A procedure for the fluorimetric determination of flunixin in the Flunex drug product (NITA-FARM, Saratov, Russia) was developed. The linearity range of the calibration curve was from 1 × 10–7 to 1 × 10–4 M, r 2 = 0.991, and the limit of detection was 4 × 10–8 M (3σ).


flunixin terbium sensitized fluorescence second ligand surfactants micelles 


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Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  • T. D. Smirnova
    • 1
  • S. N. Shtykov
    • 1
  • E. A. Zhelobitskaya
    • 1
  • M. I. Safarova
    • 2
  1. 1.Institute of ChemistryChernyshevsky Saratov National Research UniversitySaratovRussia
  2. 2.OOO NITA-FARMSaratovRussia

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