Journal of Analytical Chemistry

, Volume 72, Issue 5, pp 557–561 | Cite as

One-step in-syringe dispersive liquid–liquid microextraction and GC-FID determination of trace amounts of di(2-ethylhexyl) phthalate and its metabolite in human urine samples

  • Shahnaz Sargazi
  • Ramazan Mirzaei
  • Mashaallah Rahmani
  • Mahdi Mohammadi
  • Abdolali Khammari
  • Masoome Sheikh
Articles
  • 51 Downloads

Abstract

Di(2-ethylhexyl) phthalate (DEHP) is used as plasticizer in polyvinylchloride (PVC) plastics. Its metabolites and the parent phthalates are considered toxic. As the DEHP plasticizers are not chemically bound to PVC, they can migrate, evaporate or be leached into indoor air and atmosphere, foodstuff, and other materials. We have reported a novel, easy and available analytical method for the determination of DEHP and its metabolite, mono(2-ethylhexyl) phthalate (MEHP) in human urine samples by the in-syringe dispersive liquid–liquid microextraction method coupled with gas chromatography with flame ionization detector. The limits of detection and precision (RSD) were 2.5 μg/L and 1.4% for DEHP and 1.1 μg/L and 3.0% for MEHP, respectively. This method could be utilized for routine monitoring of the trace DEHP and MEHP in urine of human exposure to plasticizers.

Keywords

di(2-ethylhexyl) phthalate urine analysis GC-FID 

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References

  1. 1.
    Pocar, P., Fiandanese, N., Secchi, C., Berrini, A., Fischer, B., Schmidt, J.S., Schaedlich, K., and Borromeo, V., Endocrinology, 2012, vol. 153, p. 937.CrossRefGoogle Scholar
  2. 2.
    Schenkel, R. and Carl, M.P., European Union Risk Assessment Report: Bis(2-Ethylhexyl) Phthalate (DEHP), Luxembourg European Chemicals Bureau, 2008.Google Scholar
  3. 3.
    Rudel, R.A., Gray, J.M., Engel, C.L., Rawsthorne, T.W., Dodson, R.E., Ackerman, J.M., Rizzo, J., Nudelman, J.L., and Brody, J.G., Environ. Health Perspect., 2011, vol. 119, p. 914.CrossRefGoogle Scholar
  4. 4.
    Koo, H.J. and Lee, B.M., J. Toxicol. Environ. Health, 2004, vol. 67, p. 1901.CrossRefGoogle Scholar
  5. 5.
    Nerin, C., Cacho, J., and Gancedo, P., Food Addit. Contam., 1993, vol. 10, p. 453.CrossRefGoogle Scholar
  6. 6.
    Rais-Bahrami, K., Nunez, S., Revenis, M.E., Luban, N.L., and Short, B.L., Environ. Health Perspect., 2004, vol. 112, p. 1339.CrossRefGoogle Scholar
  7. 7.
    Huber, W.W., Grasl-Kraupp, B., and Schulte-Hermann, R., Crit. Rev. Toxicol., 1996, vol. 26, p. 365.CrossRefGoogle Scholar
  8. 8.
    Koch, H.M., Bolt, H.M., and Angerer, J., Arch. Toxicol., 2004, vol. 78, p. 123.CrossRefGoogle Scholar
  9. 9.
    Agency for Toxic Substances and Disease Registry, Toxicological Profile for Di(2-Ethylhexyl) Phthalate, Atlanta, GA Department of Health and Human Services, Public Health Service, 2002.Google Scholar
  10. 10.
    Dirven, H.A.A.M., Broe, P.H.H.V., and Jongeneelen, F.J., Int. Arch. Occup. Environ. Health, 1993, vol. 64, p. 55.Google Scholar
  11. 11.
    Itoh, H., Yoshida, K., and Masunaga, S., Int. J. Hyg. Environ. Health, 2005, vol. 208, p. 237.CrossRefGoogle Scholar
  12. 12.
    Ito, R., Seshimo, F., Miura, N., Kawaguchi, M., Saito, K., and Nakazawa, H., J. Pharm. Biomed. Anal., 2006, vol. 41, p. 455.CrossRefGoogle Scholar
  13. 13.
    Pietrogrande, M.C., Rossi, D., and Paganetto, G., Anal. Chim. Acta, 2003, vol. 480, p. 1.CrossRefGoogle Scholar
  14. 14.
    Suzuki, T., Yaguchi, K., and Suzuki, S., Environ. Sci. Technol., 2001, vol. 35, p. 3757.CrossRefGoogle Scholar
  15. 15.
    Sun, J.-N., Shi, Y.-P., and Chen, J., Anal. Methods, 2013, vol. 5, p. 1427.CrossRefGoogle Scholar
  16. 16.
    Wardencki, W., Curylo, J., and Namiesnik, J., Pol. J. Environ. Stud., 2005, vol. 14, p. 389.Google Scholar
  17. 17.
    Clark, J.H. and Tavener, S.J., Org. Process Res. Dev., 2007, vol. 11, p. 149.CrossRefGoogle Scholar
  18. 18.
    Capello, C., Fischer, U., and Hungerbühler, K., Green Chem., 2007, vol. 9, p. 927.CrossRefGoogle Scholar
  19. 19.
    Koller, G., Fischer, U., and Hungerbühler, K., Ind. Eng. Chem. Res., 2000, vol. 39, p. 960.CrossRefGoogle Scholar
  20. 20.
    European Committee for Standardisation, Environmental Management–Life Cycle Assessment–Principles and Framework, ENISO 14040,Brussels European Committee for Standardisation, 1997.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  • Shahnaz Sargazi
    • 1
  • Ramazan Mirzaei
    • 2
  • Mashaallah Rahmani
    • 3
  • Mahdi Mohammadi
    • 1
  • Abdolali Khammari
    • 1
  • Masoome Sheikh
    • 4
  1. 1.Health Promotion Research CenterZahedan University of Medical SciencesZahedanIran
  2. 2.Department of Occupational Health Engineering, School of HealthMashhad University of Medical ScienceMashhadIran
  3. 3.Department of Chemistry, Faculty of SciencesUniversity of Sistan and BaluchestanZahedanIran
  4. 4.Department of ChemistryZahedan Islamic Azad UniversityZahedanIran

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