Journal of Analytical Chemistry

, Volume 72, Issue 5, pp 557–561 | Cite as

One-step in-syringe dispersive liquid–liquid microextraction and GC-FID determination of trace amounts of di(2-ethylhexyl) phthalate and its metabolite in human urine samples

  • Shahnaz Sargazi
  • Ramazan Mirzaei
  • Mashaallah Rahmani
  • Mahdi Mohammadi
  • Abdolali Khammari
  • Masoome Sheikh


Di(2-ethylhexyl) phthalate (DEHP) is used as plasticizer in polyvinylchloride (PVC) plastics. Its metabolites and the parent phthalates are considered toxic. As the DEHP plasticizers are not chemically bound to PVC, they can migrate, evaporate or be leached into indoor air and atmosphere, foodstuff, and other materials. We have reported a novel, easy and available analytical method for the determination of DEHP and its metabolite, mono(2-ethylhexyl) phthalate (MEHP) in human urine samples by the in-syringe dispersive liquid–liquid microextraction method coupled with gas chromatography with flame ionization detector. The limits of detection and precision (RSD) were 2.5 μg/L and 1.4% for DEHP and 1.1 μg/L and 3.0% for MEHP, respectively. This method could be utilized for routine monitoring of the trace DEHP and MEHP in urine of human exposure to plasticizers.


di(2-ethylhexyl) phthalate urine analysis GC-FID 


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Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  • Shahnaz Sargazi
    • 1
  • Ramazan Mirzaei
    • 2
  • Mashaallah Rahmani
    • 3
  • Mahdi Mohammadi
    • 1
  • Abdolali Khammari
    • 1
  • Masoome Sheikh
    • 4
  1. 1.Health Promotion Research CenterZahedan University of Medical SciencesZahedanIran
  2. 2.Department of Occupational Health Engineering, School of HealthMashhad University of Medical ScienceMashhadIran
  3. 3.Department of Chemistry, Faculty of SciencesUniversity of Sistan and BaluchestanZahedanIran
  4. 4.Department of ChemistryZahedan Islamic Azad UniversityZahedanIran

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