One-step in-syringe dispersive liquid–liquid microextraction and GC-FID determination of trace amounts of di(2-ethylhexyl) phthalate and its metabolite in human urine samples
- 59 Downloads
Di(2-ethylhexyl) phthalate (DEHP) is used as plasticizer in polyvinylchloride (PVC) plastics. Its metabolites and the parent phthalates are considered toxic. As the DEHP plasticizers are not chemically bound to PVC, they can migrate, evaporate or be leached into indoor air and atmosphere, foodstuff, and other materials. We have reported a novel, easy and available analytical method for the determination of DEHP and its metabolite, mono(2-ethylhexyl) phthalate (MEHP) in human urine samples by the in-syringe dispersive liquid–liquid microextraction method coupled with gas chromatography with flame ionization detector. The limits of detection and precision (RSD) were 2.5 μg/L and 1.4% for DEHP and 1.1 μg/L and 3.0% for MEHP, respectively. This method could be utilized for routine monitoring of the trace DEHP and MEHP in urine of human exposure to plasticizers.
Keywordsdi(2-ethylhexyl) phthalate urine analysis GC-FID
Unable to display preview. Download preview PDF.
- 2.Schenkel, R. and Carl, M.P., European Union Risk Assessment Report: Bis(2-Ethylhexyl) Phthalate (DEHP), Luxembourg European Chemicals Bureau, 2008.Google Scholar
- 9.Agency for Toxic Substances and Disease Registry, Toxicological Profile for Di(2-Ethylhexyl) Phthalate, Atlanta, GA Department of Health and Human Services, Public Health Service, 2002.Google Scholar
- 10.Dirven, H.A.A.M., Broe, P.H.H.V., and Jongeneelen, F.J., Int. Arch. Occup. Environ. Health, 1993, vol. 64, p. 55.Google Scholar
- 16.Wardencki, W., Curylo, J., and Namiesnik, J., Pol. J. Environ. Stud., 2005, vol. 14, p. 389.Google Scholar
- 20.European Committee for Standardisation, Environmental Management–Life Cycle Assessment–Principles and Framework, ENISO 14040,Brussels European Committee for Standardisation, 1997.Google Scholar