Journal of Analytical Chemistry

, Volume 71, Issue 12, pp 1188–1197 | Cite as

Applicability of the critical chromatography concept to proteomic problems. II. Effect of mobile phase on the separation of peptides and proteins taking into account the amino acid sequence

  • A. V. Gorshkov
  • M. L. Pridatchenko
  • T. Yu. Perlova
  • I. A. Tarasova
  • L. I. Levitsky
  • M. V. Gorshkov
  • V. V. EvreinovEmail author


The effect of parameters of a binary mobile phase (chemical properties of components, gradient profile, flow rate, and pH) on the separation of peptides and proteins is theoretically investigated using a model of critical chromatography (model BioLCCC). It is demonstrated that the retention volume of peptides can be inverted by changing separation conditions.


high performance liquid chromatography proteins and peptides critical chromatography of biopolymers 


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Copyright information

© Pleiades Publishing, Ltd. 2016

Authors and Affiliations

  • A. V. Gorshkov
    • 1
  • M. L. Pridatchenko
    • 2
  • T. Yu. Perlova
    • 2
  • I. A. Tarasova
    • 2
  • L. I. Levitsky
    • 2
  • M. V. Gorshkov
    • 2
  • V. V. Evreinov
    • 1
    Email author
  1. 1.Semenov Institute of Chemical Physics, Russian Academy of SciencesMoscowRussia
  2. 2.Tal’rose Institute for Energy Problems of Chemical PhysicsMoscowRussia

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