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Determination of 2,4-dichlorophenoxyacetic acid in cereals by fluorescence polarization immunoassay

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Abstract

A fluorescence polarization immunoassay (FPIA) procedure is developed for the determination of 2,4-dichlorophenoxyacetic acid in cereal grains using a Sentry-200 portable device. We synthesized tracers, that is, antigens labeled with fluorescein derivatives, based on two fluorescent compounds and two chlorophenoxyacids. The tracer synthesized from 2,4-dichlorophenoxyacetic acid and 4-aminomethylfluorescein is found to be optimal for the FPIA of 2,4-dichlorophenoxyacetic acid. The limit of detection for 2,4-dichlorophenoxyacetic acid in flour was 40 ng/g; the analytical range is from 80 to 1000 ng/g. The percentage of recovery was 85 ± 7% at the relative standard deviation (RSD) 2–10%.

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Authors and Affiliations

  1. Department of Chemistry, Moscow State University, Moscow, 119991, Russia

    A. Yu. Boroduleva & S. A. Eremin

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  1. A. Yu. Boroduleva
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  2. S. A. Eremin
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Correspondence to A. Yu. Boroduleva.

Additional information

Original Russian Text © A.Yu. Boroduleva, S.A. Eremin, 2016, published in Zhurnal Analiticheskoi Khimii, 2016, Vol. 71, No. 9, pp. 988–994.

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Boroduleva, A.Y., Eremin, S.A. Determination of 2,4-dichlorophenoxyacetic acid in cereals by fluorescence polarization immunoassay. J Anal Chem 71, 949–954 (2016). https://doi.org/10.1134/S1061934816090045

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  • DOI: https://doi.org/10.1134/S1061934816090045

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