Identification of Barrenwort flavonoids by high-resolution tandem mass spectrometry

Abstract

Fragmentation of the main Barrenwort flavonoids—icariin, icaritin, icarisides I and II, and epimedins A and B—is studied by tandem mass spectrometry. High-resolution mass spectra of positively charged ions of these compounds are obtained under the conditions of collision-induced dissociation. Characteristic fragment ions are determined, which ensured the classification of unknown compounds as Barrenwort flavonoids. Epimedin C was isolated from raw plant material by preparative liquid chromatography; its structure was confirmed by 1H and 13C NMR spectra.

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Correspondence to O. A. Shevlyakova.

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Original Russian Text © O.A. Shevlyakova, K.Yu. Vasil’ev, A.A. Ikhalainen, A.M. Antokhin, V.F. Taranchenko, V.M. Goncharov, D.A. Mitrofanov, A.V. Aksenov, I.A. Rodin, O.A. Shpigun, 2016, published in Zhurnal Analiticheskoi Khimii, 2016, Vol. 71, No. 8, pp. 800–808.

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Shevlyakova, O.A., Vasil’ev, K.Y., Ikhalainen, A.A. et al. Identification of Barrenwort flavonoids by high-resolution tandem mass spectrometry. J Anal Chem 71, 768–776 (2016). https://doi.org/10.1134/S106193481608013X

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Keywords

  • Barrenwort
  • icariin
  • icaritin
  • icariside I and II
  • epimedin A
  • B
  • and C
  • mass spectrometry
  • fragmentation