Journal of Analytical Chemistry

, Volume 71, Issue 8, pp 768–776 | Cite as

Identification of Barrenwort flavonoids by high-resolution tandem mass spectrometry

  • O. A. Shevlyakova
  • K. Yu. Vasil’ev
  • A. A. Ikhalainen
  • A. M. Antokhin
  • V. F. Taranchenko
  • V. M. Goncharov
  • D. A. Mitrofanov
  • A. V. Aksenov
  • I. A. Rodin
  • O. A. Shpigun
Articles

Abstract

Fragmentation of the main Barrenwort flavonoids—icariin, icaritin, icarisides I and II, and epimedins A and B—is studied by tandem mass spectrometry. High-resolution mass spectra of positively charged ions of these compounds are obtained under the conditions of collision-induced dissociation. Characteristic fragment ions are determined, which ensured the classification of unknown compounds as Barrenwort flavonoids. Epimedin C was isolated from raw plant material by preparative liquid chromatography; its structure was confirmed by 1H and 13C NMR spectra.

Keywords

Barrenwort icariin icaritin icariside I and II epimedin A and C mass spectrometry fragmentation 

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Copyright information

© Pleiades Publishing, Ltd. 2016

Authors and Affiliations

  • O. A. Shevlyakova
    • 1
  • K. Yu. Vasil’ev
    • 1
  • A. A. Ikhalainen
    • 1
  • A. M. Antokhin
    • 1
  • V. F. Taranchenko
    • 1
  • V. M. Goncharov
    • 1
  • D. A. Mitrofanov
    • 1
  • A. V. Aksenov
    • 1
  • I. A. Rodin
    • 2
  • O. A. Shpigun
    • 2
  1. 1.Signal Research CenterMoscowRussia
  2. 2.Department of ChemistryMoscow State UniversityMoscowRussia

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