Abstract
A rapid, sensitive, and safe method has been proposed for the simultaneous determination of residual amounts of chloramphenicol, thiamphenicol, and chloramphenicol in food by high-performance liquid chromatography with ultraviolet detection at 228 and 280 nm using a combination of QuEChERS and dispersive liquid-liquid microextraction (DLLME). The optimum conditions of QuEChERS and DLLME sample preparation ensuring sufficient preconcentration and additional purification of the extract have been found. Recovery was 64–87% depending on the sample matrix nature. Under the selected optimum conditions, the analytical ranges taking into account the concentration coefficient equal to 10 were 0.01–5 mg/kg. The relative standard deviation of the results of analysis did not exceed 9%. The time of analysis was 1–1.5 h.
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Original Russian Text © V.G. Amelin, N.M. Volkova, N.A. Repin, T.B. Nikeshina, 2015, published in Zhurnal Analiticheskoi Khimii, 2015, Vol. 70, No. 10, pp. 1114–1120.
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Amelin, V.G., Volkova, N.M., Repin, N.A. et al. Simultaneous determination of residual amounts of amphenicols in food by HPLC with UV-detection. J Anal Chem 70, 1282–1287 (2015). https://doi.org/10.1134/S1061934815100020
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DOI: https://doi.org/10.1134/S1061934815100020