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HPLC of anthocyans with an amperometric detector: Evaluation of the antioxidant activity

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Abstract

Anthocyan complexes of a series of grape varieties with the composition traditional for Vitis vinifera, containing 3-glucosides of five anthocyanidins, delphinidin (Dp3G), cyanidin (Cy3G), petunidin (Pt3G), peonidin (Pn3G), and malvidin (Mv3G) as the major components have been studied by reversed-phase HPLC with diode-array, mass spectrometric, and amperometric detectors. A procedure has been developed for the determination of the antioxidant activity of individual anthocyans using an amperometric method; the peculiarities of using a Tsvet Yauza-01-AA system have been evaluated. It has been shown that the sensitivity of amperometric detection depends not only on the structure of anthocyans, but also on the anode voltage. A scheme of the calculation of the antioxidant activity of anthocyan complexes by the results of amperometric detection of a mixture of individual anthocyans has been proposed.

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Authors and Affiliations

  1. Department of Biology and Chemistry, Belgorod State National Research University, ul. Pobedy 85, Belgorod, 308015, Russia

    L. A. Deineka, S. L. Makarevich & V. I. Deineka

  2. Federal Center for Evaluation of Safety and Quality of Grain and Products of Its Processing, Belgorod Branch, ul. Shchorsa 8, Belgorod, 308027, Russia

    A. N. Chulkov

Authors
  1. L. A. Deineka
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  2. S. L. Makarevich
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  3. V. I. Deineka
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  4. A. N. Chulkov
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Correspondence to L. A. Deineka.

Additional information

Original Russian Text © L.A. Deineka, S.L. Makarevich, V.I. Deineka, A.N. Chulkov, 2015, published in Zhurnal Analiticheskoi Khimii, 2015, Vol. 70, No. 8, pp. 870–876.

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Deineka, L.A., Makarevich, S.L., Deineka, V.I. et al. HPLC of anthocyans with an amperometric detector: Evaluation of the antioxidant activity. J Anal Chem 70, 989–994 (2015). https://doi.org/10.1134/S1061934815080079

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  • DOI: https://doi.org/10.1134/S1061934815080079

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