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Journal of Analytical Chemistry

, Volume 70, Issue 7, pp 819–824 | Cite as

Simultaneous detection and quantification of zeatin and kinetin in coconut water using ultra performance liquid chromatography coupled with a simple step solid phase extraction

  • C. M. Y. Mooi
  • S. P. Koh
  • K. LongEmail author
Articles
  • 162 Downloads

Abstract

Trans-zeatin and kinetin are two cytokinin family members that exhibit an anti-aging effect on human fibroblast cells. These cytokinins can be found in coconut water and in the xylem sap of most plants. This study describes the first attempt of a simultaneous separation of zeatin and kinetin using a simple C18 solid phase extraction (SPE) method coupled with ultra performance liquid chromatography (UPLC). This method provided high recoveries of 95.2 and 98.6% for trans-zeatin and kinetin, respectively. Malayan Green Dwarf, a local coconut variety, was found to contain 14 μg/L trans-zeatin and 4 μg/L kinetin. This method will be useful for comprehensive research on different coconut varieties and determining the contents of cytokinins in coconut water.

Keywords

cytokinins kinetin zeatin solid phase extraction coconut water 

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Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  1. 1.Innovation and Commercialisation Division, Ministry of Science, Technology and Innovation (MOSTI) Level 3, Block C4, Complex CFederal Government Administrative CentrePutrajayaMalaysia
  2. 2.Biotechnology Research CentreMalaysian Agricultural Research and Development Institute (MARDI)Kuala LumpurMalaysia

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