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A chromatographic-mass spectrometric method for the determination of moxifloxacin in blood plasma for pharmacokinetic studies

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Abstract

A method for the determination of moxifloxacin in human blood plasma has been developed and validated by reversed-phase HPLC on a YMC-Triart C18 (150 × 2.0 mm, 3 μm) column with mass spectrometric detection. Sample preparation has been performed by solid-phase extraction on Waters Sep-Pak cartridges. The recovery of moxifloxacin was 90 ± 3%. A quadrupole mass analyzer with electrospray ionization has been used for detection at m/z 402.2 and 362.2 for moxifloxacin and ofloxacin (internal standard), respectively. The lower limit of the analytical range was 1 ng/mL. The developed method has been used for the pharmacokinetic study of moxifloxacin.

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Authors and Affiliations

  1. St. Petersburg State University, Universitetskii pr. 26, Petrodvorets, St. Petersburg, 198504, Russia

    I. S. Yaroshenko

  2. Dr. Reddy’s Laboratories Ltd, Representative Office, Ovchinnikovskaya nab. 20, Moscow, 115035, Russia

    A. Ya. Khaimenov

  3. Analytical Spectrometry Center, St. Petersburg State Polytechnic University, Gzhatskaya ul. 27, St. Petersburg, 195220, Russia

    A. V. Grigor’ev & A. A. Sidorova

Authors
  1. I. S. Yaroshenko
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  2. A. Ya. Khaimenov
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  3. A. V. Grigor’ev
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  4. A. A. Sidorova
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Corresponding author

Correspondence to I. S. Yaroshenko.

Additional information

Original Russian Text © I.S. Yaroshenko, A.Ya. Khaimenov, A.V. Grigor’ev, A.A. Sidorova, 2015, published in Zhurnal Analiticheskoi Khimii, 2015, Vol. 70, No. 7, pp. 745–753.

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Yaroshenko, I.S., Khaimenov, A.Y., Grigor’ev, A.V. et al. A chromatographic-mass spectrometric method for the determination of moxifloxacin in blood plasma for pharmacokinetic studies. J Anal Chem 70, 860–868 (2015). https://doi.org/10.1134/S1061934815050160

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  • DOI: https://doi.org/10.1134/S1061934815050160

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