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Enantioseparation of terbutaline by online concentration capillary electrophoresis coupling with partial filling technique

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Abstract

A novel, simple and sensitive method for the enantioseparation of terbutaline enantiomers was developed using capillary zone electrophoresis (CZE) in combination with partial filling technique and field-amplified sample concentration. Under optimal conditions, the racemic terbutaline was resolved in less than 21 min by partially filling a fused-silica capillary with 15 mM β-cyclodextrin (β-CD, 1.9 psi, 90 s) and carrying out an electrophoresis with 50 mM phosphate buffer (pH 1.8) at 15 kV separation voltage. The sensitivity was further improved by using field-amplified sample injection (0.2 psi, 2.5 s water plug). Compared with conventional CZE, the resolution and sensitivity of two enantiomers were much improved (the detection limit decreased by 18–25-fold).

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Mianyang Normal University, Mianyang, 621000, P.R. China

    Shuya Cui, Xiaoli Hu & Juan Li

  2. School of Physics and Chemistry, Xihua University, Chengdu, 610039, P.R. China

    Jiaqin Liu

Authors
  1. Shuya Cui
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  2. Jiaqin Liu
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  3. Xiaoli Hu
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  4. Juan Li
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Correspondence to Xiaoli Hu.

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Cui, S., Liu, J., Hu, X. et al. Enantioseparation of terbutaline by online concentration capillary electrophoresis coupling with partial filling technique. J Anal Chem 70, 81–86 (2015). https://doi.org/10.1134/S1061934815010165

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  • DOI: https://doi.org/10.1134/S1061934815010165

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