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Liquid chromatography of some steroid hormones in aqueous-organic, micellar, and cyclodextrin mobile phases

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Abstract

The retention of hydrocortisone, progesterone, dexamethasone, and prednisolone is studied by HPLC and thin-layer chromatography using aqueous-organic, micellar, and cyclodextrin mobile phases. It is demonstrated that micellar and cyclodextrin mobile phases improve the efficiency and selectivity of the chromatographic separation of the indicated hormones compared to aqueous-organic eluents. By comparing the numbers of theoretical plates, the heights equivalent to a theoretical plate, and resolution it was found that micellar mobile phases are more efficient than cyclodextrin ones and can be used for work with Sorbfil plates.

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Authors and Affiliations

  1. Institute of Chemistry, Saratov State University, ul. Astrakhanskaya 83, Saratov, 410012, Russia

    E. G. Sumina, S. N. Shtykov & V. Z. Uglanova

  2. Department of Microbiology, Biotechnology, and Chemistry, Saratov State Agricultural University, Teatral’naya pl. 1, Saratov, 410012, Russia

    O. N. Sorokina

Authors
  1. E. G. Sumina
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  2. S. N. Shtykov
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  3. O. N. Sorokina
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  4. V. Z. Uglanova
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Corresponding author

Correspondence to S. N. Shtykov.

Additional information

Original Russian Text © E.G. Sumina, S.N. Shtykov, O.N. Sorokina, V.Z. Uglanova, 2014, published in Zhurnal Analiticheskoi Khimii, 2014, Vol. 69, No. 10, pp. 1105–1113.

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Sumina, E.G., Shtykov, S.N., Sorokina, O.N. et al. Liquid chromatography of some steroid hormones in aqueous-organic, micellar, and cyclodextrin mobile phases. J Anal Chem 69, 1009–1016 (2014). https://doi.org/10.1134/S1061934814100153

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  • DOI: https://doi.org/10.1134/S1061934814100153

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