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Determination of acysole in whole blood by high-performance liquid chromatography

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Abstract

A method is developed for the determination of a carbon monoxide antidote acysole in whole blood using hydrophilic interaction high-performance liquid chromatography with UV detection. Two methods were used for sample preparation: protein precipitation with acetonitrile and solid-phase extraction (SPE) with an acysole recovery of 95.8 and 89.8%, respectively. Chromatographic determination was performed in the isocratic mode on a Nucleodur HILIC column and with an acetonitrile: water 95: 5 mobile phase at 225 nm. The calibration graphs are linear in the concentration range 0.056–111.1 μg/mL. The limits of detection and determination of acysole were 0.050 and 0.120 μg/mL after protein precipitation with acetonitrile and 0.071 and 0.169 μg/mL with SPE, respectively.

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Authors and Affiliations

  1. Institute of Biomedical Research and Technology, Bol’shoi Tishinskii per. 43/20, Moscow, 123557, Russia

    S. V. Kursakov, V. Yu. Belov & V. I. Sevast’yanov

  2. Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, ul. Shchukinskaya 1, Moscow, 123182, Russia

    V. I. Sevast’yanov

Authors
  1. S. V. Kursakov
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  2. V. Yu. Belov
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  3. V. I. Sevast’yanov
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Corresponding author

Correspondence to S. V. Kursakov.

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Original Russian Text © S.V. Kursakov, V.Yu. Belov, V.I. Sevast’yanov, 2014, published in Zhurnal Analiticheskoi Khimii, 2014, Vol. 69, No. 8, pp. 875–879.

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Kursakov, S.V., Belov, V.Y. & Sevast’yanov, V.I. Determination of acysole in whole blood by high-performance liquid chromatography. J Anal Chem 69, 800–804 (2014). https://doi.org/10.1134/S1061934814060094

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  • DOI: https://doi.org/10.1134/S1061934814060094

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