Abstract
A method is developed for the determination of a carbon monoxide antidote acysole in whole blood using hydrophilic interaction high-performance liquid chromatography with UV detection. Two methods were used for sample preparation: protein precipitation with acetonitrile and solid-phase extraction (SPE) with an acysole recovery of 95.8 and 89.8%, respectively. Chromatographic determination was performed in the isocratic mode on a Nucleodur HILIC column and with an acetonitrile: water 95: 5 mobile phase at 225 nm. The calibration graphs are linear in the concentration range 0.056–111.1 μg/mL. The limits of detection and determination of acysole were 0.050 and 0.120 μg/mL after protein precipitation with acetonitrile and 0.071 and 0.169 μg/mL with SPE, respectively.
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References
Luzhnikov, E.A., Klinicheskaya toksikologiya (Clinical Toxicology), Moscow: Meditsina, 1982.
Riddex, L. and Dellgar, U., Prehosp. Disaster Med., 2001, vol. 16, p. 50.
Babaniyazov, Kh.Kh, Barinov, V.A., and Nechiporenko, S.P., Uniform Russia, 2005, no. 8, p. 116.
Basok, Yu.B., Alekseeva, O.S., Salomatina, L.A., Zaitseva, M.A., and Sevastianov, V.I., Rare Metals, 2009, vol. 28, p. 521.
Sevast’yanov, V.I., Salomatina, L.A., Kuznetsova, E.G., Seregina, M.V., and Basok, Yu.B., Perspektivnye Materialy, 2008, no. 6, p. 55.
Lisetskaya, L.G., Cand. Sci. (Biol) Dissertation, Irkutsk: Inst. Gigieny Truda i Professional’nykh Zabolevanii SO RAMN, 1995.
Fedorova, G.A., Cand. Sci. (Chem) Dissertation, Irkutsk: Limnologicheskii Inst. SO RAN, 2003.
Alpert, A.J., J. Chromatogr., 1990, vol. 499, p. 177.
Sychev, K.S. and Styskin, I.E., Analitika, 2012, no. 4, p. 56.
Doerffel, K., Statistik in der analytische chemie, Leipzig: Deutsche Verlag fur Grundstoffindustrie, 1990.
Eksperiandova, L.P., Belikov, K.N., Khimchenko, S.V., and Blank, T.A., J. Anal. Chem., 2010, vol. 65, no. 3, p. 223.
Guidance for Industry. Bioanalytical Method Validation, Rockville, 2001.
Guideline on Validation of Bioanalytical Methods, London, 2009.
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Original Russian Text © S.V. Kursakov, V.Yu. Belov, V.I. Sevast’yanov, 2014, published in Zhurnal Analiticheskoi Khimii, 2014, Vol. 69, No. 8, pp. 875–879.
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Kursakov, S.V., Belov, V.Y. & Sevast’yanov, V.I. Determination of acysole in whole blood by high-performance liquid chromatography. J Anal Chem 69, 800–804 (2014). https://doi.org/10.1134/S1061934814060094
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DOI: https://doi.org/10.1134/S1061934814060094