Skip to main content

Simultaneous determination of several mycotoxins by rapid immunofiltration assay

Abstract

A method is developed for the simultaneous rapid determination of three mycotoxins, zearalenone (ZEN), ochratoxin A (OTA), and fumonisin B1 (Fum) by membrane immunofiltration analysis using a marker enzyme of alkaline phosphatase (AP) and two mycotoxins, deoxynivalenol (DON) and total T-2 toxin (T2) and HT-2 toxin (HT2) with horseradish peroxidase (HRP). The analysis is based on a competitive interaction between the antigene, free and bound to the enzyme, and antibodies immobilized on a membrane. The procedures of membrane fabrication and the conditions of mycotoxin to determination in model mixtures and extracts from wheat, corn, and silage are optimized. The influence of sample preparation on the results of analysis is studied. It is shown that the additives of polymers favor the reduction of the matrix effect in the analysis of complex matrixes using conjugated HRP. The methods developed allow the determination of mycotoxins at a level of the maximal permissible concentrations legislated by EU directives. The corresponding values (μg/kg) are 50, 2.5, and 500 in wheat; 100, 2.5, and 500 in corn; and 125, 25, and 1250 in silage for the simultaneous quantification of ZEN, OTA, and Fum (AP marker). For the determination of DON and total T2/HT2 with HRP, 1250 (1000) and 100 (500) in wheat and corn(silage). The procedures were validated by the analysis of spiked and naturally contaminated samples. The analysis of 10 samples takes 25 min.

This is a preview of subscription content, access via your institution.

References

  1. Zolotov, Yu.A., Ivanov, V.M., and Amelin, V.G., Khimicheskie test-metody analiza (Rapid Chemical Tests), Moscow: Editorial URSS, 2002, vol. 304.

    Google Scholar 

  2. Problemy analiticheskoi khimii. T. 12: Biokhimicheskie metody analiza (Problem of Analytical Chemistry. Vol. 12: Biochemical Analytical Methods), Dzantiev, B.B., Ed., Moscow: Bach Inst. Biochemistry, 2010.

    Google Scholar 

  3. Xiang, X., Tianping, W., and Zhigang, T., J. Immunol. Method., 2003, vol. 280, nos. 1–2, p. 49.

    CAS  Article  Google Scholar 

  4. Zhang, C., Zhang, Y., and Wang, S., J. Agric. Food Chem., 2006, vol. 54, no. 7, p. 2502.

    CAS  Article  Google Scholar 

  5. Ballesteros, B., Barcelo, D., Dankwardt, A., Schneider, P., and Marco, M.P., Anal. Chim. Acta, 2003, vol. 475, nos. 1–2, p. 105.

    CAS  Article  Google Scholar 

  6. Goryacheva, I.Yu., Rusanova, T.Yu., Burmistrova, N.A., and De Saegep, S., J. Anal. Chem., 2009, vol. 64, no. 8, p. 768.

    CAS  Article  Google Scholar 

  7. Urusov, A.E., Zherdev, A.V., and Dzantiev, B.B., Prikl. Biokhim. Mikrobiol, 2010, vol. 46, no. 3, p. 276.

    CAS  Google Scholar 

  8. Sibanda, L., De Saeger, S., Van Peteghem, C., Grabarkiewicz-Szczesna, J., and Tomczak, M., J. Agric. Food Chem., 2000, vol. 48, no. 12, p. 5864.

    CAS  Article  Google Scholar 

  9. Saha, D., Acharya, D., Roy, D., Shrestha, D., and Dhar, T.K., Anal. Chim. Acta, 2007, vol. 584, no. 2, p. 343.

    CAS  Article  Google Scholar 

  10. Saha, D., Acharya, D., and Dhar, T.K., Anal. Bioanal. Chem., 2006, vol. 385, no. 5, p. 847.

    CAS  Article  Google Scholar 

  11. Rusanova, T.Yu., Beloglazova, N.V., Goryacheva, I.Yu., Lobeau, M., Van Peteghem, C., and De Saeger, S., Anal. Chim. Acta, 2009, vol. 653, no. 1, p. 97.

    CAS  Article  Google Scholar 

  12. Goryacheva, I.Yu., Rusanova, T.Yu., Beloglazova, N.V., Voronov, I.I., and De Saeger, S., J. Anal. Chem., 2010, vol. 65, no. 7, p. 760.

    CAS  Article  Google Scholar 

  13. Pal, A., Acharya, D., Saha, D., Roy, D., and Dhar, T.K., J. Food Protection, 2005, vol. 68, no. 10, p. 2169.

    CAS  Google Scholar 

  14. Kolosova, A.Yu., De Saeger, S., Eremin, S.A., and Van Peteghem, C., Anal. Bioanal. Chem., 2007, vol. 387, no. 3, p. 1095.

    Article  Google Scholar 

  15. Bhat, R., Rai, R.V., and Karim, A.A., Comp. Rev. Food Sci. Food Safety, 2010, vol. 9, no. 1, p. 57.

    CAS  Article  Google Scholar 

  16. Paepens, C., De Saeger, S., Sibanda, L., Barna-Vetro, I., Leglise, I., Van Hove, F., and Van Peteghem, C., Anal. Chim. Acta, 2004, vol. 523, no. 2, p. 229.

    CAS  Article  Google Scholar 

  17. Pal, A. and Dhar, T.K., Anal. Chem., 2004, vol. 76, no. 1, p. 98.

    CAS  Article  Google Scholar 

  18. Sibanda, L., De Saeger, S., and Van Peteghem, C., Int. J. Food Microbiol, 1999, vol. 48, no. 3, p. 203.

    CAS  Article  Google Scholar 

  19. Pal, A., Acharya, D., Saha, D., and Dhar, T.K., Anal. Chem., 2004, vol. 76, no. 14, p. 4237.

    CAS  Article  Google Scholar 

  20. Burmistrova, N.A., Goryacheva, I.Yu., Basova, E.Yu., Franki, A.-S., Elewaut, D., Van Beneden, K., Deforce, D., Van Peteghem, C., and De Saeger, S., Anal. Bioanal. Chem., 2009, vol. 395, no. 5, p. 1301.

    CAS  Article  Google Scholar 

  21. Collin, R., Schneider, E., Briggs, L., and Towers, N., Food Agric. Immunol, 1998, vol. 10, no. 2, p. 91.

    CAS  Article  Google Scholar 

  22. Ricciardi, C., Castagna, R., Ferrante, I., Frascella, F., Marasso, S.L., Ricci, A., Canavese, G., Lore, A., Prelle, A., Gullino, M.L., and Spadaro, D., Biosens. Bioelectron., 2013, vol. 40, no. 1, p. 233.

    CAS  Article  Google Scholar 

  23. Wang, Y., Liu, N., Ning, B., Liu, M., Lv, Z., Sun, Z., Peng, Y., Chen, C., Li, J., and Gao, Z., Biosens. Bioelectron., 2012, vol. 34, no. 1, p. 44.

    Article  Google Scholar 

  24. Won-Bo, S., Dzantiev, B.B., Eremin, S.A., and Chung, D.H., J. Microbiol. Biotechnol, 2009, vol. 19, no. 1, p. 83.

    Google Scholar 

  25. Huang, Z.B., Xu, Y., Li, L.S., Li, Y.P., Zhang, H., and He, Q.H., Food Control, 2012, vol. 28, no. 1, p. 7.

    CAS  Article  Google Scholar 

  26. Njumbe, EdiageE., Di Mavungu, J., Goryacheva, I.Y., Van Peteghem, C., and De Saeger, S., Anal. Bioanal. Chem., 2012, vol. 403, no. 1, p. 265.

    Article  Google Scholar 

  27. Schneider, E., Usleber, E., Maertlbauer, E., Dietrich, R., and Terplan, G., Food. Add. Contam., 1995, vol. 12, no. 3, p. 387.

    CAS  Article  Google Scholar 

  28. He, Q.H., Xu, Y., Wang, D., Kang, M., Huang, Z.B., and Li, Y.P., Food Chem., 2012, vol. 134, no. 1, p. 507.

    CAS  Article  Google Scholar 

  29. Hermanson, G.T., Bioconjugate Techniques, Academic Press, 1996.

    Google Scholar 

  30. Monbaliu, S., Development of Multi-Mycotoxin LC-MS/MS Methods and Application in Food and Feed Analysis, PhD thesis, Ghent: Ghent University, 2011.

    Google Scholar 

  31. De Saeger, S. and Van Peteghem, C., J. Food Prot., 1999, vol. 62, no. 1, p. 65.

    Google Scholar 

  32. Krska, R., Welzig, E., and Boudra, H., Animal Feed Sci. Tech., 2007, vol. 137, nos. 3–4, p. 241.

    CAS  Article  Google Scholar 

  33. De Girolamo, A., Solfrizzo, M., von Holst, Ch., and Visconti, A., Food. Add. Contam., 2001, vol. 18, no. 1, p. 59.

    Article  Google Scholar 

  34. Trullols, E., Ruisanchez, I., and Rius, F.X., Trend. Anal. Chem., 2004, vol. 23, no. 2, p. 137.

    CAS  Article  Google Scholar 

  35. EC Commission Decision 657/2002, Official Journal of the European Communities, L 221/8.

  36. Cavaliere, C., D’Ascenzo, G., Foglia, P., Pastorini, E., Samperi, R., and Lagana, A., Food Chem., 2005, vol. 92, no. 3, p. 559.

    CAS  Article  Google Scholar 

  37. Maragos, M. and Mecormick, P., Food Agric. Immunol, 2000, vol. 12, no. 3, p. 181.

    CAS  Article  Google Scholar 

  38. De Saeger, S., Sibanda, L., Paepens, S., Lobeau, M., Delmulle, B., Barna-Vetro, I., and Van Peterghem, C., Mycotoxin Res., 2006, vol. 22, no. 2, p. 100.

    Article  Google Scholar 

  39. Veselova, I.A., Kireiko, A.V., and Shekhovtsova, T.N., Prikl. Biokhim. Mikrobiol., 2009, vol. 45, no. 2, p. 143.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to N. A. Burmistrova.

Additional information

Original Russian Text © N.A. Burmistrova, T.Yu. Rusanova, N.A. Yurasov, S. De Saeger, I.Yu. Goryacheva, 2014, published in Zhurnal Analiticheskoi Khimii, 2014, Vol. 69, No. 6, pp. 586–595.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Burmistrova, N.A., Rusanova, T.Y., Yurasov, N.A. et al. Simultaneous determination of several mycotoxins by rapid immunofiltration assay. J Anal Chem 69, 525–534 (2014). https://doi.org/10.1134/S1061934814060045

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1061934814060045

Keywords

  • mycotoxins
  • rapid assays
  • membrane immunofiltration analysis
  • horseradish peroxidase
  • alkaline phosphatase