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Antioxidant activity and phenolic compounds of buckwheat and barley by the data of spectrophotometry and HPLC

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Abstract

The antioxidant activity of buckwheat and barley extracts by reaction with 1,1-diphenyl-2-picrylhydrazyl and the total of phenolic compounds have been determined using the Folin-Ciocalteu reagent. It has been found that water-ethanol extracts of buckwheat are characterized by higher antioxidant activity (6.2 ± 0.5 μM-eq. of Trolox/g) and concentration of phenolic compounds (4.41 ± 0.07 mg-eq. of rutin/g) compared to barley extracts (4.2 ± 0.3 μM-eq. of Trolox/g and 2.4 ± 0.1 mg-eq. of rutin/g, respectively). A series of phenolic compounds have been identified by HPLC with UV detection and mass spectrometric detection with electrospray ionization. The main phenolic compounds-antioxidants in buckwheat extracts are rutin, catechin and epicatechin, 1-O-caffeoyl-O-rutinoside (m/z 487), and epicatechin-O-3,4-dimethylgallate (m/z 469), and in the barley extract, catechin, prodelphinidin B3 (m/z 593), procyanidin B3 (m/z 577), and procyanidin C2 (m/z 865).

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Authors and Affiliations

  1. Institute of Chemistry, Saratov State University, Saratov, ul. Astrakhanskaya 83, 410012, Russia

    S. S. Aleksenko

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  1. S. S. Aleksenko
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Original Russian Text © S.S. Aleksenko, 2013, published in Zhurnal Analiticheskoi Khimii, 2013, Vol. 68, No. 5, pp. 505–513.

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Aleksenko, S.S. Antioxidant activity and phenolic compounds of buckwheat and barley by the data of spectrophotometry and HPLC. J Anal Chem 68, 458–465 (2013). https://doi.org/10.1134/S106193481305002X

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  • DOI: https://doi.org/10.1134/S106193481305002X

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