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Preconcentration of methylxanthines on hyper-cross-linked polystyrene followed by their determination by high-performance liquid chromatography

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Abstract

We studied the possibility of hyper-cross-linked polystyrene application to the dynamic sorption preconcentration (solid-phase extraction) of methylxanthines (caffeine, theophylline, theobromine, diprophylline, and pentoxifylline) from aqueous solutions. The conditions of preconcentration were optimized as follows: solution volume of 25 mL (pH ∼ 6), solution flow rate 0.7 mL/min, microcolumn size 25 × 2.7 mm, adsorbent weight 0.055 g. The compounds were desorbed into a 0.5-mL portion of methanol and determined in the eluate by reversed-phase HPLC with spectrophotometric detection at 280 nm. Sorption preconcentration provided more than 30-fold reduction of methylxanthine detection limits. The detection limits for methylxanthines are 1 (theophylline, theobromine), 2 (caffeine, diprophylline) and 4 (pentoxifylline) ng/mL. The procedure was applied to the analysis of urine-based model mixtures.

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Authors and Affiliations

  1. Department of Chemistry, Moscow State University, Moscow, 119991, Russia

    S. G. Dmitrienko, E. Yu. Andreeva, V. V. Tolmacheva & Yu. A. Zolotov

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  1. S. G. Dmitrienko
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  2. E. Yu. Andreeva
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  3. V. V. Tolmacheva
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  4. Yu. A. Zolotov
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Original Russian Text © S.G. Dmitrienko, E.Yu. Andreeva, V.V. Tolmacheva, Yu.A. Zolotov, 2013, published in Zhurnal Analiticheskoi Khimii, 2013, Vol. 68, No. 2, pp. 108–112.

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Dmitrienko, S.G., Andreeva, E.Y., Tolmacheva, V.V. et al. Preconcentration of methylxanthines on hyper-cross-linked polystyrene followed by their determination by high-performance liquid chromatography. J Anal Chem 68, 95–99 (2013). https://doi.org/10.1134/S106193481302007X

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  • DOI: https://doi.org/10.1134/S106193481302007X

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