Advertisement

Journal of Analytical Chemistry

, Volume 67, Issue 11, pp 925–929 | Cite as

A simple and rapid detection of viral protein using RNA oligonucleotide in a biosensor

  • Changhyun Roh
  • Sang-Eun Kim
  • Sung-Kee Jo
Articles

Abstract

The monitoring of non-structural viral protein 3 (NS3) has been of considerable interest in developing simple and reliable methods for detection of hepatitis C virus (HCV) for applications in diagnostic medicine. Although enzyme-linked immunosorbent assay (ELISA) is the most general method in HCV detection, using antibody brings problems. This method is temperature-sensitive and requires specific reactions condition. In addition, secondary antibody conjugated with enzyme and fluorescent dye is required. To overcome these bottlenecks, we designed a streptavidin-biotin conjugation method, namely, the RNA oligonucleotide sensor system that could monitor viral protein with detection limit of 500 pg/mL by using biotin-tagged RNA oligonucleotide in forteBio’s Octet optical biosensor system. In this study, we proposed an efficient method for simple and convenient detection of HCV viral protein, with the advantage of target specific monitoring.

Keywords

hepatitis C virus (HCV) viral protein streptavidin-biotin conjugation RNA oligonucleotide octet optical biosensor 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Pawlotsky, J.M., Curr. Top. Microbiol. Immunol., 2006, vol. 299, p. 261.CrossRefGoogle Scholar
  2. 2.
    Reed, K.E. and Rice, C.M., Curr. Top. Microbiol. Immunol., 2000, vol. 242, p. 55.CrossRefGoogle Scholar
  3. 3.
    Kwong, A.D., Kim, J.L., and Lin, C., Curr. Top. Microbiol. Immunol., 2000, vol. 242, p. 171.CrossRefGoogle Scholar
  4. 4.
    Kim, D.W., Gwack, Y., Han, J.H., and Choe, J., Biochem. Biophys. Res. Commun., 1995, vol. 215, p. 160.CrossRefGoogle Scholar
  5. 5.
    Kanai, A., Tanabe, K., and Kohara, M., FEBS Lett., 1995, vol. 376, p. 221.CrossRefGoogle Scholar
  6. 6.
    Gaudy, C., Thevenas, C., Tichet, J., Mariotte, N., Goudeau, A., and Dubois, F., J. Clin. Microbiol., 2005, vol. 43, p. 1722.CrossRefGoogle Scholar
  7. 7.
    Cullen, B.R., Science, 2007, vol. 317, p. 329.CrossRefGoogle Scholar
  8. 8.
    Abdiche, Y., Malashock, D., Pinkerton, A., and Pons, J., Anal. Biochem., 2008, vol. 377, p. 209.CrossRefGoogle Scholar
  9. 9.
    Rich, R.L., and Myszka, D.G., Anal. Biochem., 2007, vol. 361, p. 1.CrossRefGoogle Scholar
  10. 10.
    Farkas, A.M., Kilgore, T.M., and Lotze, M.T., Curr. Opin. Invest. Drugs, 2007, vol. 8, p. 981.Google Scholar
  11. 11.
    Laemmli, U.K., Nature, 1970, vol. 227, p. 680.CrossRefGoogle Scholar
  12. 12.
    Bradford, M.A., Anal. Biochem., 1976, vol. 72, p. 248.CrossRefGoogle Scholar
  13. 13.
    Lotze, M.T., US Patent 2009/0147264A1.Google Scholar
  14. 14.
    Sarrazin, C., Kieffer, T.L., Bartels, D., Hanzelka, B., Muh, U., Welker, M., Wincheringer, D., Zhou, Y., Chu, H.M., Lin. C., Weegink, C., Reesink, H., Zeuzem, S., and Kwong, A.D., Gastroenterology, 2007, vol. 132, p. 1767.CrossRefGoogle Scholar
  15. 15.
    Lamarre, D., Anderson, P.C., Bailey, M., Beaulieu, P., Bolger, G., Bonneau, P., Bos, M., Cameron, D.R., Cartier, M., Cordingley, M.G., Faucher, A.M., Goudreau, N., Kawai, S.H., Kukolj, G., Lagace, L., LaPlante, S.R., Narjes, H., Poupart, M.A., Rancourt, J., Sentjens, R.E., St. George, R., Simoneau, B., Steinmann, G., Thibeault, D., Tsantrizos, Y.S., Weldon, S.M., and Yong, C.L., Nature, 2003, vol. 426, p. 186.CrossRefGoogle Scholar
  16. 16.
    Roh, C., Lee, H.Y., Kim, S.E., and Jo, S.K., J. Chem. Tech. Biotech., 2010, vol. 85, p. 1130.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • Changhyun Roh
    • 1
  • Sang-Eun Kim
    • 2
  • Sung-Kee Jo
    • 1
  1. 1.Radiation Research Division for Biotechnology, Advanced Radiation Technology Institute (ARTI)Korea Atomic Energy Research Institute (KAERI)JeonbukRepublic of Korea
  2. 2.Department of Nuclear MedicineSeoul National University College of Medicine, Seoul National University Bundang HospitalSeongnamRepublic of Korea

Personalised recommendations