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System peaks and optimization of anion separation in capillary electrophoresis with non-reversed electroosmotic flow

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Abstract

We studied system peaks present in the electropherograms obtained in the separation of anions by capillary electrophoresis with indirect spectrophotometric detection and cathode electroosmotic flow (EOF) with a chromate background electrolyte. The system peaks correspond to the zones with changed concentration of the background electrolyte; they formed when the zones of each analyte passed through the outlet of the capillary and then moved towards the EOF detector. It has been revealed that the height and area of the system peaks linearly depends on the concentration of the corresponding anion and the areas of the system peaks can achieve 10% of the anion peak area. An algorithm has been proposed for the determination of the optimal conditions for anion separation using hydrodynamic pressure for the regulation of the EOF flow rate. This algorithm prevents the overlapping of the anion and system peaks.

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Authors and Affiliations

  1. Institute of Chemistry and Chemical Technology, Siberian Branch, Russian Academy of Sciences, ul. K. Marksa 42, Krasnoyarsk, 660049, Russia

    V. V. Sursyakova, S. N. Kalyakin, G. V. Burmakina & A. I. Rubaylo

  2. Department of Chemistry and Metallurgy, Siberian Federal University, pr. Svobodnyi 79, Krasnoyarsk, 660041, Russia

    G. V. Burmakina & A. I. Rubaylo

Authors
  1. V. V. Sursyakova
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  2. S. N. Kalyakin
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  3. G. V. Burmakina
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  4. A. I. Rubaylo
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Additional information

Original Russian Text © V.V. Sursyakova, S.N. Kalyakin, G.V. Burmakina, A.I. Rubaylo, 2012, published in Zhurnal Analiticheskoi Khimii, 2012, Vol. 67, No. 9, pp. 871–877.

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Sursyakova, V.V., Kalyakin, S.N., Burmakina, G.V. et al. System peaks and optimization of anion separation in capillary electrophoresis with non-reversed electroosmotic flow. J Anal Chem 67, 783–789 (2012). https://doi.org/10.1134/S1061934812090080

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  • DOI: https://doi.org/10.1134/S1061934812090080

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