Journal of Analytical Chemistry

, Volume 67, Issue 9, pp 783–789 | Cite as

System peaks and optimization of anion separation in capillary electrophoresis with non-reversed electroosmotic flow

  • V. V. Sursyakova
  • S. N. Kalyakin
  • G. V. Burmakina
  • A. I. Rubaylo


We studied system peaks present in the electropherograms obtained in the separation of anions by capillary electrophoresis with indirect spectrophotometric detection and cathode electroosmotic flow (EOF) with a chromate background electrolyte. The system peaks correspond to the zones with changed concentration of the background electrolyte; they formed when the zones of each analyte passed through the outlet of the capillary and then moved towards the EOF detector. It has been revealed that the height and area of the system peaks linearly depends on the concentration of the corresponding anion and the areas of the system peaks can achieve 10% of the anion peak area. An algorithm has been proposed for the determination of the optimal conditions for anion separation using hydrodynamic pressure for the regulation of the EOF flow rate. This algorithm prevents the overlapping of the anion and system peaks.


capillary electrophoresis system peaks optimization of anion separation non-reversed electroosmotic flow indirect detection 


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Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • V. V. Sursyakova
    • 1
  • S. N. Kalyakin
    • 1
  • G. V. Burmakina
    • 1
    • 2
  • A. I. Rubaylo
    • 1
    • 2
  1. 1.Institute of Chemistry and Chemical Technology, Siberian BranchRussian Academy of SciencesKrasnoyarskRussia
  2. 2.Department of Chemistry and MetallurgySiberian Federal UniversityKrasnoyarskRussia

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