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Journal of Analytical Chemistry

, Volume 67, Issue 9, pp 778–782 | Cite as

Determination of hydroxycarboxylic acids in food products by capillary electrophoresis

  • A. M. Golubenko
  • V. V. Nikonorov
  • T. G. Nikitina
Articles

Abstract

A procedure for the capillary-electrophoretic determination of lactic, malic, tartaric, and citric acids in food products was developed. The use of 3-nitrobenzoic acid as a light-absorbing component of the running buffer was proposed for the indirect photometric detection of these substances. This considerably increased the determination sensitivity (up to n × 10 μg/L), as compared with currently available analogs. The composition of the running buffer was optimized: 3-nitrobenzoic acid, 10 mM; cetyltrimethylammonium bromide, 0.5 mM; EDTA, 0.1 mM; and monoethanolamine, to pH 5.3. The procedure was tested with the samples of food products: fruits, juices, nectars, wines, beer, etc. The accuracy of the analytical results was confirmed by the standard addition method.

Keywords

hydroxycarboxylic acids capillary electrophoresis food products 

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Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • A. M. Golubenko
    • 1
  • V. V. Nikonorov
    • 1
  • T. G. Nikitina
    • 1
  1. 1.Department of ChemistrySt. Petersburg State UniversitySt. PetersburgRussia

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