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Journal of Analytical Chemistry

, Volume 67, Issue 9, pp 790–794 | Cite as

Determination of zearalenone and ochratoxin a mycotoxins in grain by fluorescence polarization immunoassay

  • A. P. Bondarenko
  • S. A. Eremin
Articles

Abstracts

Rapid procedures are reported for the determination of zearalenone and ochratoxin A, which make possible the analysis of up to 10 grain samples within 20 min by fluorescence polarization immunoassay. The influence of various fluorescein-based tracers on the sensitivity of analysis is examined. The most promising was admitted the use of ethylenediamine thiocarbamoylfluorescein and aminomethylfluorescein. The detection limits of the method for zearalenone and ochratoxin A were 3 and 1.5 ng/mL for a model system and 15 and 10 μg/kg for grain, respectively. The performed analysis of spiked grain samples demonstrated the recovery of mycotoxins at a level of 84–97%.

Keywords

mycotoxins zearalenone ochratoxin A FPIA 

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References

  1. 1.
    Dandliker, W.B., Kelly, K.J., Dandliker, J., and Levin, J., Immunochemistry, 1973, vol. 10, p. 219.CrossRefGoogle Scholar
  2. 2.
    Jameson, D.M. and Ross, J.A., Chem. Rev., 2010, vol. 110, p. 2685.CrossRefGoogle Scholar
  3. 3.
    Complementary Immunoassays, Collins, W.P., Ed., New York: Wiley, 1988.Google Scholar
  4. 4.
    Lacowicz, J.R., Principles of Fluorescence Spectroscopy, New York: Springer, 2006, 3rd ed.CrossRefGoogle Scholar
  5. 5.
    Eccleston, J.F., Hutchinson, J.P., and Jameson, D.M., Prog. Med. Chem., 2005, vol. 43, p. 20.Google Scholar
  6. 6.
    Zinedine, A., Soriano, J.M., Molto, J.C., and Manes, J., Food Chem. Toxicol., 2007, vol. 45, p. 1.CrossRefGoogle Scholar
  7. 7.
    Khabriev, R.U. and Kaletina, N.I., Toksikologicheskaya khimiya. Analiticheskaya toksikologiya (Toxicological Chemistry. Analytical Toxicology), Moscow: GEOTAR-Media, 2010.Google Scholar
  8. 8.
    European Mycotoxin Regulations and Proposal. http://www.mycotoxins.info
  9. 9.
    SanPiN (Sanitary Rulesand Norms) 2.3.2.1078-01: Hygienic Requirements for the Safety and Nutritional Value of Foodstuffs, 2001.Google Scholar
  10. 10.
    Maragos, C., Toxins, 2009, vol. 1, p. 196.CrossRefGoogle Scholar
  11. 11.
    Liu, B.H., Tsao, Z.J., Wang, J.J., and Yu, F.Y., Anal. Chem., 2008, vol. 80, p. 7029.CrossRefGoogle Scholar
  12. 12.
    Thongrussamee, T., Kuzmina, N.S., Shim, W.-B., Jiratpong, T., Eremin, S.A., Intrasook, J., and Chung, D.-H., Food Addit. Contam., 2008, vol. 25, p. 1944.Google Scholar
  13. 13.
    Chu, F.S., Hsia, M.T.S., and Sun, P., J. Assoc. Off. Anal. Chem., 1977, vol. 60, p. 791.Google Scholar
  14. 14.
    Burkin, A.A., Kononenko, G.P., Soboleva, N.A., and Zotova, E.V., Appl. Biochem. Microbiol., 2000, vol. 36, no. 3, p. 282.CrossRefGoogle Scholar
  15. 15.
    Zezza, F., Longobardi, F., Pascale, M., Eremin, S.A., and Visconti, A., Anal. Bioanal. Chem., 2009, vol. 395, p. 1317.CrossRefGoogle Scholar
  16. 16.
    Chun, H.S., Choi, E.H., Chang, H.J., Choi, S.W., and Eremin, S.A., Anal. Chim. Acta, 2009, vol. 639, p. 83.CrossRefGoogle Scholar
  17. 17.
    Rodbard, D., J. Anal. Biochem., 1978, vol. 90, p. 1.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • A. P. Bondarenko
    • 1
  • S. A. Eremin
    • 1
  1. 1.Department of ChemistryLomonosov Moscow State UniversityMoscowRussia

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