Skip to main content
SpringerLink
Log in

Pharmacokinetic study of metoprolol in rabbit plasma by capillary electrophoresis with laser-induced Fluorescence detection

  • Articles
  • Published:
Journal of Analytical Chemistry Aims and scope Submit manuscript

Abstract

This work described a sensitive method for determination of metoprolol in rabbit plasma. The method involved purification by ultrafiltration, derivatization with Fluorescein isothiocyanate, separation by capillary electrophoresis and determination by laser-induced fluorescence detector. Other components in plasma including a variety of amino acids and proteins did not interfere with the determination of metoprolol under experimental conditions. The assay had a wide range (2.0–500 ng/mL) of linearity and a detection limit of 0.8 ng/mL. The intra- and inter-day precisions of the QC samples were satisfactory with RSD less than 10% and accuracy within 10%. This method was successfully applied to pharmacokinetic study of metoprolol in rabbit blood.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ceniceros, C., Maguregui, M.I., Jimenez, R.M., and Alonso, R.M., J. Chromatogr. B, 1998, vol. 705, no. 1, p. 97.

    Article  CAS  Google Scholar 

  2. Kim, K.H., Lee, J.H., Ko, M.Y., Shin, K.S., Kang, J.S., Mar, W.C., and Youm, J.R., Chromatographia, 2002, vol. 55, p. 81.

    Article  Google Scholar 

  3. Yilmaz, B., Arslan, S., and Akba, V., Talanta, 2009, vol. 80, p. 346.

    Article  CAS  Google Scholar 

  4. Boralli, V.B., Coelho, E.B., Cerqueira, P.M., and Lanchote, V.L., J. Chromatogr. B, 2005, vol. 823, no. 2, p. 195.

    Article  CAS  Google Scholar 

  5. Johnson, R.D. and Lewis, R.J., Forensic. Sci. Int., 2006, vol. 156, no. 2–3, p. 106.

    Article  CAS  Google Scholar 

  6. Gowda, K.V., Mandal, U., Selvan, P.S., Solomon, W.D.S., Ghosh, A., Sarkar, A.K., Agarwal, S., Rao, T.N., and Pal, T.K., J. Chromatogr. B, 2007, vol. 858, p. 13.

    Article  CAS  Google Scholar 

  7. Al Azzam, K., Elbashir, A.A., Elbashir, M.A., Saad, B., and Abdul Hamid, S., Anal. Lett., 2009, vol. 42, p. 1458.

    Article  CAS  Google Scholar 

  8. Balesteros, M.R., Faria, A.F., and Oliveira, A.L., J. Brazil. Chem. Soc., 2007, vol. 18, p. 554.

    Article  CAS  Google Scholar 

  9. Wren Stephen, A.C. and Rowe Raymond C., J. Chromatogr. A, 1993, vol. 635, p. 113.

    Article  Google Scholar 

  10. Chen, B., Du, Y., and Li, P., Electrophoresis, 2009, vol. 30, p. 2747.

    Article  CAS  Google Scholar 

  11. Chen, B. and Du, Y., J Chromatogr. A, 2010, vol. 1217, p. 1806.

    Article  CAS  Google Scholar 

  12. Gagyi, L., Gyéresi, á., and Kilár, F., J. Biochem. Bioph. Meth., 2008, vol. 70, p. 1268.

    Article  CAS  Google Scholar 

  13. Park, K.L., Kim, K.H., Jung, S.H., Lim, H.M., Hong, C.H., and Kang, J.S., J. Pharmaceut Biomed., 2002, vol. 27, p. 569.

    Article  CAS  Google Scholar 

  14. Zhang, Z.X. and He, Y.Z., J. Chromatogr. A, 2005, vol. 1066, p. 211.

    Article  CAS  Google Scholar 

  15. Lim, H.K., Linh, P.T., Hong, C.H., Kim, K.H., and Kang, J.S., J. Chromatogr. B, 2001, vol. 755, p. 259.

    Article  CAS  Google Scholar 

  16. Wang, W., Zhang, Y., Wang, J., Shi, X. and Ye, J., Meat Sci., 2010, vol. 85, p. 302.

    Article  CAS  Google Scholar 

  17. Lu, H., Lin, J., Yu, L., Xu, L., and Chen, G., Electrophoresis, 2008, vol. 29, p. 3588.

    Article  Google Scholar 

  18. Li, Y.M., Qu, Y., Vandenbussche, E., Arckens, L., and Vandesande, F., J. Neurosci. Meth., 2001, vol. 105, p. 211.

    Article  CAS  Google Scholar 

  19. Paproski, R.E., Roy, K.I., and Lucy, C.A., J. Chromatogr. A, 2002, vol. 946, p. 265.

    Article  CAS  Google Scholar 

  20. Cheng, F.C., Chen, Y.T., Kuo, J.S., Chen, S.H., and Chang, L.C., J. Pharmaceut. Biomed., 1996, vol. 14, p. 1169.

    Article  CAS  Google Scholar 

  21. Guidance for Industry, Bioanalytical Method Validation, US Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research (CDER), 2001.

Download references

Author information

Authors and Affiliations

  1. College of Environmental Science and Engineering, Chang’an University, Xi’an, Shannxi, 710054, China

    Yuyun Chen

  2. Education School of Nationalities, Shaanxi Normal University, Xi’an, 710062, China

    Weiping Yang

Authors
  1. Yuyun Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Weiping Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

The article is published in the original.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chen, Y., Yang, W. Pharmacokinetic study of metoprolol in rabbit plasma by capillary electrophoresis with laser-induced Fluorescence detection. J Anal Chem 67, 572–576 (2012). https://doi.org/10.1134/S1061934812060202

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1061934812060202

Keywords

Search

Navigation