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Journal of Analytical Chemistry

, Volume 66, Issue 14, pp 1417–1422 | Cite as

Detection of nerve agent markers by liquid chromatography-mass spectrometry

  • I. A. RodinEmail author
  • A. V. Braun
  • I. A. Anan’eva
  • O. A. Shpigun
  • E. I. Savel’eva
  • I. V. Rybal’chenko
  • S. L. Bolotov
  • G. M. Rodchenkov
Articles

Abstract

An approach to the detection of metabolites of organophosphorous agents (OPA), such as O-iso-propylmethylphosphonic acid (detection limit, 4 ng/mL), O-pinacolylmethylphosphonic acid (0.6 ng/mL), and O-isobutylmethylphosphonic acid (1 ng/mL), in plasma samples was developed using liquid chromatography-mass spectrometry. The curves of the elutionxcretion of OPA metabolites were obtained for the samples of biological material of rats exposed to toxic substances. Determination was performed by mass spectrometry with electrospray ionization in the negative ion mode, using deprotonated molecules for detection. The biological samples were analyzed by reversed-phase chromatography using hydrophilic end-capped adsorbents. Solid phase extraction on reversed-phase adsorption cartridges containing a copolymer of styrene and divinylbenzene was proposed for sample preparation.

Keywords

high performance liquid chromatography-mass spectrometry electrospray ionization detection nerve agents 

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References

  1. 1.
    Konventsiya o zapreshchenii razrabotki, proizvodstva, nakopleniya i primeneniya khimicheskogo oruzhiya i o ego unichtozhenii (The Convention on the Prohibition of the Development, Production, Stockpiling, and Use of Chemical Weapons and on Their Destruction), Geneva, 1993.Google Scholar
  2. 2.
    Kataoka, M. and Seto, Y., J. Chromatogr. B, 2003, vol. 795, no. 1, p. 123.CrossRefGoogle Scholar
  3. 3.
    Rybal’chenko, I.V., Khlebnikova, N.S., Savel’eva, E.I., Radilov, A.S., and Rembovskii, V.R., Ros. Khim. Zhurn., 2005, vol. 49, no. 2, p. 26.Google Scholar
  4. 4.
    Black, R.M. and Read, R.W., J. Chromatogr. A, 1997, vol. 759, no. 2, p. 79.CrossRefGoogle Scholar
  5. 5.
    Hayes, T.H., Kenny, D.V., and Hernon-Kenny, L., J. Med. Chem. Def., 2004, vol. 2, no. 1, p. 121.Google Scholar
  6. 6.
    Noort, D., Hulst, A.G., Platenburg, D.H., and Polhuijs, M., Arch. Toxicol., 1998, vol. 72, no. 10, p. 671.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • I. A. Rodin
    • 1
    Email author
  • A. V. Braun
    • 1
  • I. A. Anan’eva
  • O. A. Shpigun
    • 1
  • E. I. Savel’eva
    • 2
  • I. V. Rybal’chenko
    • 3
  • S. L. Bolotov
    • 4
  • G. M. Rodchenkov
    • 4
  1. 1.Department of ChemistryMoscow State UniversityMoscowRussia
  2. 2.Scientific-Research Institute of Hygiene, Occupational Pathology, and Human EcologyRussian Ministry of HealthVsevolzhskii region, Leningrad oblastRussia
  3. 3.Lyumekszashchita Research Production CompanyMoscowRussia
  4. 4.Anti Doping CenterMinistry of Sport, Tourism, and Youth PolicyMoscowRussia

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