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Immunochromatographic assay for the detection of ochratoxin A

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Abstract

The detection of mycotoxins—toxic contaminants of fungal origin—is an important problem in the food and feed quality control. An immunochromatographic system was developed for the detection of ochratoxin A (OTA), which is one of the priority contaminants in grain. Monoclonal antibodies against OTA and their conjugates with colloidal gold nanoparticles were prepared. The detection is based on the competition of OTA in a sample and an OTA-protein conjugate immobilized on a test strip for the binding to anti-bodies on the colloidal particle surface. The method was tested in the analysis of plant extracts (maize and barley extracts). It was shown that OTA can be detected in a medium with a high content of an organic solvent (up to 35% of methanol). The disappearance of the line in the test zone is visually detected at OTA concentrations starting from 50 ng/mL. In the case of the video-digital detection of changes in the color intensity of the test zone, the limit of detection of OTA is 5 ng/mL. The duration of the assay is 10 min.

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Authors and Affiliations

  1. Institute of Biochemistry, Russian Academy of Sciences, Leninskii prosp. 33, Moscow, 119071, Russia

    A. E. Urusov, S. N. Kostenko, A. V. Zherdev & B. B. Dzantiev

  2. Russian Center of Molecular Diagnostics and Therapy, Simferopolskii bulv. 8, Moscow, 117630, Russia

    P. G. Sveshnikov

Authors
  1. A. E. Urusov
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  2. S. N. Kostenko
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  3. P. G. Sveshnikov
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  4. A. V. Zherdev
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  5. B. B. Dzantiev
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Additional information

Original Russian Text © A.E. Urusov, S.N. Kostenko, P.G. Sveshnikov, A.V. Zherdev, B.B. Dzantiev, 2011, published in Zhurnal Analiticheskoi Khimii, 2011, Vol. 66, No. 8, pp. 884–890.

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Urusov, A.E., Kostenko, S.N., Sveshnikov, P.G. et al. Immunochromatographic assay for the detection of ochratoxin A. J Anal Chem 66, 770–776 (2011). https://doi.org/10.1134/S1061934811080144

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  • DOI: https://doi.org/10.1134/S1061934811080144

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