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Simultaneous determination of six free fatty acids in alcohol extract of P. japonicus C. A. Mey. van major (Burk.) C. Y. Wu et K. M. Feng by gas chromatography-mass spectrometry

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Abstract

P. japonicus C. A. Mey. var. major (Burk.) C. Y. Wu et K. M. Feng is an important medicinal plant and has special beneficial effects on human health. Six types of free fatty acids (FFAs) were identified and quantified in the alcohol extract of P. japonicus C. A. Mey. var. major with a GC-MS method, including two fatty acids essential to the human body. All the six FFAs identified in alcohol extract were quantified using nonadecanoic acid as an internal standard. The results showed that the alcohol extract was abundant in four types of FFAs, unsaturated FFAs amounted to 47.04% of the total FFA content, and there were no trans FFAs. Palmitic acid (38.82%, 4.74 ± 0.09 mg/g) was the predominant fatty acid, followed by linoleic acid (27.92%, 2.31 ± 0.07 mg/g), stearic acid (14.14%, 1.54 ± 0.04 mg/g) and oleic acid (11.04%, 1.02 ± 0.03 mg/g).

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References

  1. Park, J.D., Rhee, D.K., and Lee, Y.H., Phytochem. Rev., 2005, vol. 4, p. 159.

    Article  CAS  Google Scholar 

  2. Wang, C.Z., McEntee, E., Wicks, S., Wu, J.A., and Yuan, C.S., J. Nat. Med., 2006, vol. 60, no. 2, p. 97.

    Article  CAS  Google Scholar 

  3. Ma, X.Q., Xiao, H.B., and Liang, X.M., Chromatographia, 2006, vol. 64, nos. 1–2, p. 31.

    Article  CAS  Google Scholar 

  4. Goswami, R.S. and Punja, Z.K., Physiol. Mol. Plant Pathol., 2008, vol. 72, nos. 1–3, p. 10.

    Article  CAS  Google Scholar 

  5. Pharmacopoeia of the People’s Republic of China, Beijing: Chemical Industry, 2005.

  6. Yamahara, J., Kubomura, Y., Miki, K., and Fujimura, H., J. Ethnopharmacol., 1987, vol. 19, no. 1, p. 95.

    Article  CAS  Google Scholar 

  7. Briskin, D.P., Plant Physiol., 2000, vol. 124, p. 507.

    Article  CAS  Google Scholar 

  8. Zhao, R., Zhao, Y., Li, D.M., Wang, J.Y., and Zeng, Y., Modern Chin. Med., 2008, vol. 10, no. 7, p. 3.

    CAS  Google Scholar 

  9. Bao, X., Zhan, C.S., and Xiao, Z.Y.J., West Chin. Med., 1992, vol. 7, no. 1, p. 49.

    Google Scholar 

  10. Skonberg, D.I. and Perkins, B.L., Food Chem., 2002, vol. 77, no. 4, p. 401.

    Article  CAS  Google Scholar 

  11. Makoto, M. and de Souza, N.E., Food Chem., 2005, vol. 93, no. 3, p. 445.

    Article  Google Scholar 

  12. Sajid, M., Ilkay, O., Zaheer, A., Sinem, A., and Muhammad, G., Food Chem., 2008, vol. 109, no. 4, p. 855.

    Article  Google Scholar 

  13. Phillip, A., Mark, S., Matt, B., Charles, K., Brian, S., and Hasson, A.S., Atm. Environ., 2008, vol. 42, no. 26, p. 6417.

    Article  Google Scholar 

  14. Halpern, B., Pereira, W.E., Solomon, M.D., and Steed, E., Anal. Biochem., 1971, vol. 39, no. 1, p. 156.

    Article  CAS  Google Scholar 

  15. Wallace, F.A., Neely, S.J., Miles, E.A., and Calder, P.C., Immunol. Cell Biol., 2000, vol. 78, no. 1, p. 40.

    Article  CAS  Google Scholar 

  16. Cherif, S., Frikhaa, F., Gargouria, Y., and Miled, N., Food Chem., 2008, vol. 111, no. 4, p. 930.

    Article  CAS  Google Scholar 

  17. Calder, P.C., Nutr. Rev., 1998, vol. 56, no. 1, p. 70.

    Article  Google Scholar 

  18. Nutritional Aspects of Cardiovascular Disease, London: HMSO, 1994.

  19. Ascherio, A., Katan, M.B., Zock, P.L., Stampfer, M.J., and Willett, W.C.N., Engl. J. Med., 1999, vol. 340, no. 25, p. 1994.

    Article  CAS  Google Scholar 

  20. Craig-Schmidt, M.C., Holzer, B.M., and Chow, C.K., in Fatty Acids in Foods and Their Health Implications, New York: Marcel Dekker, 2000, 2nd ed., p. 307.

    Google Scholar 

  21. Zhao, J., Li, S.P., Yang, F.Q., Li, P., and Wang, Y.T.J., Chromatogr. A, 2006, vol. 1108, no. 2, p. 188.

    Article  CAS  Google Scholar 

  22. Hartman, L. and Lago, R.C.A., Lab. Pract., 1973, vol. 22, no. 8, p. 475.

    CAS  Google Scholar 

  23. Destaillats, F. and Cristina, C.H.J., J. Chromatogr. A, 2007, vol. 1169, nos. 1–2, p. 175.

    Article  CAS  Google Scholar 

  24. Yi, L.Z., He, J., Liang, Y.Z., Yuan, D.L., Gao, H.Y., and Zhou, H.H., Chem. Phys. Lipids, 2007, vol. 150, no. 2, p. 204.

    Article  CAS  Google Scholar 

  25. Metcalfe, L.D., Schmitz, A.A., and Pelka, J.R., Anal. Chem., 1966, vol. 38, no. 3, p. 514.

    Article  CAS  Google Scholar 

  26. Blau, K. and King, G.S., Handbook of Derivatives for Chromatography, London: Heyden and Son, 1978.

    Google Scholar 

  27. Rosenfeld, J.M., Anal. Chim. Acta, 2002, vol. 465, nos. 1–2, p. 93.

    Article  CAS  Google Scholar 

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Zhang, Y.N., Yue, X.F. & Wang, Z.Z. Simultaneous determination of six free fatty acids in alcohol extract of P. japonicus C. A. Mey. van major (Burk.) C. Y. Wu et K. M. Feng by gas chromatography-mass spectrometry. J Anal Chem 65, 1068–1072 (2010). https://doi.org/10.1134/S1061934810100138

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