Abstract
P. japonicus C. A. Mey. var. major (Burk.) C. Y. Wu et K. M. Feng is an important medicinal plant and has special beneficial effects on human health. Six types of free fatty acids (FFAs) were identified and quantified in the alcohol extract of P. japonicus C. A. Mey. var. major with a GC-MS method, including two fatty acids essential to the human body. All the six FFAs identified in alcohol extract were quantified using nonadecanoic acid as an internal standard. The results showed that the alcohol extract was abundant in four types of FFAs, unsaturated FFAs amounted to 47.04% of the total FFA content, and there were no trans FFAs. Palmitic acid (38.82%, 4.74 ± 0.09 mg/g) was the predominant fatty acid, followed by linoleic acid (27.92%, 2.31 ± 0.07 mg/g), stearic acid (14.14%, 1.54 ± 0.04 mg/g) and oleic acid (11.04%, 1.02 ± 0.03 mg/g).
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Park, J.D., Rhee, D.K., and Lee, Y.H., Phytochem. Rev., 2005, vol. 4, p. 159.
Wang, C.Z., McEntee, E., Wicks, S., Wu, J.A., and Yuan, C.S., J. Nat. Med., 2006, vol. 60, no. 2, p. 97.
Ma, X.Q., Xiao, H.B., and Liang, X.M., Chromatographia, 2006, vol. 64, nos. 1–2, p. 31.
Goswami, R.S. and Punja, Z.K., Physiol. Mol. Plant Pathol., 2008, vol. 72, nos. 1–3, p. 10.
Pharmacopoeia of the People’s Republic of China, Beijing: Chemical Industry, 2005.
Yamahara, J., Kubomura, Y., Miki, K., and Fujimura, H., J. Ethnopharmacol., 1987, vol. 19, no. 1, p. 95.
Briskin, D.P., Plant Physiol., 2000, vol. 124, p. 507.
Zhao, R., Zhao, Y., Li, D.M., Wang, J.Y., and Zeng, Y., Modern Chin. Med., 2008, vol. 10, no. 7, p. 3.
Bao, X., Zhan, C.S., and Xiao, Z.Y.J., West Chin. Med., 1992, vol. 7, no. 1, p. 49.
Skonberg, D.I. and Perkins, B.L., Food Chem., 2002, vol. 77, no. 4, p. 401.
Makoto, M. and de Souza, N.E., Food Chem., 2005, vol. 93, no. 3, p. 445.
Sajid, M., Ilkay, O., Zaheer, A., Sinem, A., and Muhammad, G., Food Chem., 2008, vol. 109, no. 4, p. 855.
Phillip, A., Mark, S., Matt, B., Charles, K., Brian, S., and Hasson, A.S., Atm. Environ., 2008, vol. 42, no. 26, p. 6417.
Halpern, B., Pereira, W.E., Solomon, M.D., and Steed, E., Anal. Biochem., 1971, vol. 39, no. 1, p. 156.
Wallace, F.A., Neely, S.J., Miles, E.A., and Calder, P.C., Immunol. Cell Biol., 2000, vol. 78, no. 1, p. 40.
Cherif, S., Frikhaa, F., Gargouria, Y., and Miled, N., Food Chem., 2008, vol. 111, no. 4, p. 930.
Calder, P.C., Nutr. Rev., 1998, vol. 56, no. 1, p. 70.
Nutritional Aspects of Cardiovascular Disease, London: HMSO, 1994.
Ascherio, A., Katan, M.B., Zock, P.L., Stampfer, M.J., and Willett, W.C.N., Engl. J. Med., 1999, vol. 340, no. 25, p. 1994.
Craig-Schmidt, M.C., Holzer, B.M., and Chow, C.K., in Fatty Acids in Foods and Their Health Implications, New York: Marcel Dekker, 2000, 2nd ed., p. 307.
Zhao, J., Li, S.P., Yang, F.Q., Li, P., and Wang, Y.T.J., Chromatogr. A, 2006, vol. 1108, no. 2, p. 188.
Hartman, L. and Lago, R.C.A., Lab. Pract., 1973, vol. 22, no. 8, p. 475.
Destaillats, F. and Cristina, C.H.J., J. Chromatogr. A, 2007, vol. 1169, nos. 1–2, p. 175.
Yi, L.Z., He, J., Liang, Y.Z., Yuan, D.L., Gao, H.Y., and Zhou, H.H., Chem. Phys. Lipids, 2007, vol. 150, no. 2, p. 204.
Metcalfe, L.D., Schmitz, A.A., and Pelka, J.R., Anal. Chem., 1966, vol. 38, no. 3, p. 514.
Blau, K. and King, G.S., Handbook of Derivatives for Chromatography, London: Heyden and Son, 1978.
Rosenfeld, J.M., Anal. Chim. Acta, 2002, vol. 465, nos. 1–2, p. 93.
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Zhang, Y.N., Yue, X.F. & Wang, Z.Z. Simultaneous determination of six free fatty acids in alcohol extract of P. japonicus C. A. Mey. van major (Burk.) C. Y. Wu et K. M. Feng by gas chromatography-mass spectrometry. J Anal Chem 65, 1068–1072 (2010). https://doi.org/10.1134/S1061934810100138
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DOI: https://doi.org/10.1134/S1061934810100138