Journal of Analytical Chemistry

, Volume 65, Issue 3, pp 267–275 | Cite as

Physicochemical constants as a factor determining the need for the derivatization of organic substances in analysis by gas chromatography

  • I. G. Zenkevich


A criterion was proposed to estimate the necessity of the derivatization of organic substances for their determination on conventional nonpolar phases, based on such characteristic of analytes as molecular weight (M r), normal boiling point (T bp), and molar refraction (MR D). All these constants can be presented as indices relative to nonpolar n-alkanes (similarly to chromatographic retention indices), I(M), I(T), and I(MR D), which can be compared to each other as differences ΔT − M = I(T) − I(M) and ΔT − M R D = I(T) − I(MR D). Substances do not require derivatization if ΔT − M < 400 and ΔT − M R D < 600, while at ΔT − M > 600 and ΔT − MRD > 800, derivatization is necessary.


Retention Index Molar Refraction Normal Boiling Point Physicochemical Constant Nonpolar Phasis 
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© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • I. G. Zenkevich
    • 1
  1. 1.Faculty of ChemistrySt. Petersburg State UniversitySt. PetersburgRussia

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