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Determination of Alachlor in food products by a fluorescence polarization immunoassay

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Abstract

A procedure for the determination of Alachlor in liquid media using a fluorescence polarization immunoassay (FPIA) was developed. The effects of the structure and purification degree of a tracer (analyte with a fluorescent label) on the analytical signals, sensitivity, and selectivity in the determination of Alachlor were studied. The tracer-antibody binding constants (K T) and antigen-antibody binding constants (affinity constants K Af) were calculated, and optimum immunoreagent pairs for the determination of Alachlor were chosen. The calibration graph was linear over the Alachlor concentration range 0.01–1.0 µg/mL; the detection limit was 8 ng/mL. The procedure was tested in the analysis of model solutions and soy-containing food products (sausages).

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Authors and Affiliations

  1. Lipetsk Sate Technical University, ul. Moskovskaya 30, Lipetsk, 398600, Russia

    Yu. V. Nartova & T. N. Ermolaeva

  2. Lipetsk Regional Bureau of Forensic Medical Examination, ul. Grishina 17, Lipetsk, 398017, Russia

    M. R. Fleisher

  3. King’s College London, 150 Stamford Street, London, SE1 9NH, UK

    R. Abuknesha

  4. Moscow Sate University, Moscow, 119992, Russia

    S. A. Eremin

Authors
  1. Yu. V. Nartova
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  2. T. N. Ermolaeva
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  3. M. R. Fleisher
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  4. R. Abuknesha
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  5. S. A. Eremin
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Additional information

Original Russian Text © Yu.V. Nartova, T.N. Ermolaeva, M.R. Fleisher, R. Abuknesha, S.A. Eremin, 2008, published in Zhurnal Analiticheskoi Khimii, 2008, Vol. 63, No. 5, pp. 546–552.

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Nartova, Y.V., Ermolaeva, T.N., Fleisher, M.R. et al. Determination of Alachlor in food products by a fluorescence polarization immunoassay. J Anal Chem 63, 499–505 (2008). https://doi.org/10.1134/S106193480805016X

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  • DOI: https://doi.org/10.1134/S106193480805016X

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