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Rapid titrimetric and spectrophotometric assay methods for the determination of lamivudine in pharmaceuticals using iodate and two dyes

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Abstract

One titrimetric and two spectrophotometric methods, which are simple, sensitive and rapid, are described for the assay of lamivudine in bulk drug and in tablet dosage forms using potassium iodate and two dyes, methyl orange and indigocarmine, as reagents. In titrimetry, an aqueous solution of lamivudine is titrated directly with iodate in an acidic medium, and in the presence of an excess of bromide using methyl orange as an indicator. After the decoloration of the red color of methyl orange, the residual bromine is titrated iodometrically to a starch endpoint. Spectrophotometric methods involve the addition of a known excess of iodate in an acidic medium and in the presence of an excess of bromide followed by the determination of residual bromine by the reaction with a fixed amount of either methyl orange and measuring the absorbance at 520 nm (method A), or indigo carmine and measuring the absorbance at 610 nm (method B). In all methods, the amount of iodate which reacted corresponds to the amount of lamivudine content. The titrimetric method is applicable over the 1.5–8.0 mg range. The systems obey Beer’s law for 0.5–5.0 µg/mL (method A) and 1.25–12.5 μg/mL (method B). The calculated apparent molar absorptivity values are found to be 3.3 × 104 and 9.3 × 103 L mol−1 cm−1, for method A and method B, respectively, and the corresponding Sandell sensitivity values are 6.94 and 24.62 ng/cm2. The limits of detection and quantification are also reported for both spectrophotometric methods. Intra-and interday precision and accuracy for the developed methods have been evaluated. The methods were successfully applied to the assay of lamivudine in tablet form and the results were compared with those of a reference method by applying the Student’s t-test and F-test. No interference was observed from common tablet adjuvants. The accuracy and reliability of the methods were further ascertained by recovery experiments using the standard addition technique.

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References

  1. Martindale: The Complete Drug Reference, Parfitt, K., Ed., London: Pharmaceutical Press, 1999, 32nd ed.

    Google Scholar 

  2. Aymard, G., Legrand, M., Trichereau, N., and Diquet, B., J. Chromatogr. B, 2000, vol. 744, no. 2, p. 227.

    Article  CAS  Google Scholar 

  3. Pereira, A.S., Kenney, K.B., Cohen, M.S., Hall, J.E., Eron, J.J., Tridewell, R.R., and Dunn, J.A., J. Chromatogr. B, 2000, vol. 742, no. 1, p. 173.

    Article  CAS  Google Scholar 

  4. Fan, B. and Stewert, J.T., J. Pharm. Biomed. Anal., 2002, vol. 28, no. 5, p. 903.

    Article  CAS  Google Scholar 

  5. Hoetelmanur, R.M.W., Profit, M., Mecnhorst, P.L., Mulder, J.W., and Beijnen, J.H., J. Chromatogr. B, 1998, vol. 713, no. 2, p. 387.

    Article  Google Scholar 

  6. Ozkan, S.A. and Uslu, B., J. Liq. Chromatogr. Rel. Technol., 2002, vol. 25, p. 1447.

    Article  CAS  Google Scholar 

  7. Moore, J.D., Valette, G., Darque, A., Zhon, X.J., and Sommadossi, J.P., J. Am. Soc. Mass Spectrom., 2000, vol. 11, no. 12, p. 1134.

    Article  CAS  Google Scholar 

  8. Plumb, R.S., Gray, R.D.M., Hasker, A.J., and Taylor, S., J. Chromatogr. B, 1996, vol. 687, no. 2, p. 457.

    Article  CAS  Google Scholar 

  9. Zheng, J.J., Wu, S.T., and Emm, J.A., J. Chromatogr. B 2001, vol. 761, no. 2, p. 195.

    Article  CAS  Google Scholar 

  10. Fan, B. and Stewart, J.T., J. Liq. Chromatogr. Rel. Technol., 2002, vol. 25, no. 2, p. 241.

    Article  CAS  Google Scholar 

  11. Pai, N.R. and Desai, A.S., Indian Drugs, 2003, vol. 40, p. 111.

    CAS  Google Scholar 

  12. Palled, M.S., Rajesh, P.M.N., Chatter, M., and Bhat, A.R., Indian J. Pharm. Sci., 2005, vol. 67, no. 1, p. 110.

    CAS  Google Scholar 

  13. Uslu, B. and Ozkan, S.A., Anal. Chim. Acta, 2002, vol. 466, no. 1, p. 175.

    Article  CAS  Google Scholar 

  14. Wankhede, S.B., Gupta, K.R., and Wadodkar, S.G., Indian J. Pharm. Sci., 2005, vol. 67, no. 1, p. 96.

    CAS  Google Scholar 

  15. Rajasekaran, A., Murugesan, S., Rajagopal, K.A., Blessing, A.G.I., Chakravarthy, T.N., and Pushparaj, D.S.I., Oriental J. Chem., 2000, vol. 16, p. 563.

    CAS  Google Scholar 

  16. Sankar, D.G., Reddy, M.V.V.N., Kumar, J.M.R., and Murthy, T.K., Asian J. Chem., 2002, vol. 14, no. 1, p. 433.

    CAS  Google Scholar 

  17. Baig, M.V., Kapse, G.S., and Raju, S.A., Asian J. Chem., 2002, vol. 14, no. 1, p. 185.

    Google Scholar 

  18. Sarma, G.N., Sastry, C.K., and Sastry, C.S.P., Asian J. Chem., 2002, vol. 14, no. 2, p. 683.

    CAS  Google Scholar 

  19. Sarma, C.S.N., Sastry, C.K., and Sastry, C.S.P., Acta Cienc. Indica Chem., 2002, vol. 28, p. 221.

    Google Scholar 

  20. Appalaraju, S., Karadi, A.B., Kamalapurkar, O.S., and Sarasambi, P.S., Asian J. Chem., 2002, vol. 14, no. 1, p. 475.

    CAS  Google Scholar 

  21. Bassett, J., Denny, R.C., Jeffery, G.H., and Mendham, J., Vogel’s Text Book of Quantitative Inorganic Analysis, London: Longman, 1978, 4th ed., p. 376.

    Google Scholar 

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Basavaiah, K., Somashekar, B.C. & Ramakrishna, V. Rapid titrimetric and spectrophotometric assay methods for the determination of lamivudine in pharmaceuticals using iodate and two dyes. J Anal Chem 62, 542–548 (2007). https://doi.org/10.1134/S1061934807060081

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  • DOI: https://doi.org/10.1134/S1061934807060081

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