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Journal of Analytical Chemistry

, Volume 61, Issue 3, pp 295–299 | Cite as

Rapid determination of subnanogram urapidil using flow injection enhancement chemiluminescence

  • Q. Yue
  • Z. Song
  • C. Wang
Articles

Abstract

A sensitive flow-injection (FI) chemiluminescence (CL) for the determination of urapidil is described in this paper. It is based on the enhancement effect of urapidil on the CL reaction between luminol and hydrogen peroxide. The increment of CL intensity is proportional to the concentration of urapidil in the range 0.1−10 ng/mL (R2=0.9986), with a detection limit (3σ) of 0.03 ng/mL. The whole process, at a flow rate of 2.0 mL/min, including sampling and washing, could be completed in 0.5 min, and the relative standard deviation (RSD) at the concentration of 0.1, 1.0, and 10.0 ng/mL was less than 3.0% (n = 5). The proposed method has been successfully applied for the determination of urapidil in pharmaceutical preparation, human urine, and serum.

Keywords

Hydrogen Standard Deviation Analytical Chemistry Hydrogen Peroxide Detection Limit 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Pleiades Publishing, Inc. 2006

Authors and Affiliations

  • Q. Yue
    • 1
  • Z. Song
    • 1
  • C. Wang
    • 1
  1. 1.Department of ChemistryNorthwest UniversityXi’anChina

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