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Preparation of Nanodiamond–Lysozyme–Miramistin Composite and Prospects of Its Application in Heart Valve Prosthetics

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Abstract

Creation of biocompatible coatings for xenogenic materials that can be used to manufacture prosthetic heart valves is an urgent and, unfortunately, still unsolved problem. It is necessary to obtain a biomaterial that would comply with the mechanical characteristics of a human valve and possess antimicrobial properties, which are of critical importance during the first postsurgical days. Biocompatible coatings can be used for this purpose, and it has turned out that detonation nanodiamonds are suitable for their preparation. The developed functional surface of nanodiamonds allows them to adsorb antibiotics; nanodiamonds are nontoxic and do not cause additional calcification. In this study, we have proposed to prepare a composite coating composed of nanodiamonds, lysozyme, and miramistin as broad-spectrum antimicrobial agents. The use of tritium-labeled nanodiamonds has made it possible to study the distribution of nanodiamond–lysozyme complexes after intravenous administration to mice and showed that the majority of the material remains at the place of injection. It has been shown that nanodiamond–lysozyme–miramistin composites exhibit strong antimicrobial activity, while the nanodiamond–miramistin complex shows no toxicity with respect to Staphylococcus aureus. Thus, the nanodiamond–lysozyme–miramistin composite can be used to create coatings for materials of prosthetic heart valves.

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Funding

This work was supported by the Russian Science Foundation (project no. 22-23-00019).

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Authors and Affiliations

  1. Department of Chemistry, Moscow State University, 119991, Moscow, Russia

    M. G. Chernysheva, G. A. Badun & A. G. Popov

  2. Bakulev National Medical Research Center of Cardiovascular Surgery, 121552, Moscow, Russia

    I. S. Chashchin & N. M. Anuchina

  3. Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, 119334, Moscow, Russia

    I. S. Chashchin

  4. Petrov National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, 197758, St. Petersburg, Russia

    A. V. Panchenko

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  1. M. G. Chernysheva
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  4. I. S. Chashchin
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  5. N. M. Anuchina
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Correspondence to M. G. Chernysheva.

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ETHICS APPROVAL AND CONSENT TO PARTICIPATE

All experiments with mice were performed according to the Appendix A “Guidelines for care and use of laboratory animals,” European convention on the protection of vertebrate animals used in experiments or for other scientific purposes (ETS no. 123). The protocol of the study was approved by the Local Ethic Committee of the Petrov National Medical Research Center of Oncology (no. 14 of October 30, 2018).

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The authors of this work declare that they have no conflicts of interes-t.

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Translated by A. Kirilin

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Chernysheva, M.G., Badun, G.A., Popov, A.G. et al. Preparation of Nanodiamond–Lysozyme–Miramistin Composite and Prospects of Its Application in Heart Valve Prosthetics. Colloid J 86, 120–129 (2024). https://doi.org/10.1134/S1061933X23600987

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